METTL4介导的N-甲基腺嘌呤DNA修饰调控拟南芥的耐热性。

METTL4-mediated N-methyladenine DNA modification regulates thermotolerance in Arabidopsis thaliana.

作者信息

Cai Linjun, Ma Ancheng, Lei Jiao, He Chongsheng

机构信息

Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation, College of Biology, Hunan University, Changsha, 410082 Hunan, China.

出版信息

Plant Sci. 2024 Jan;338:111916. doi: 10.1016/j.plantsci.2023.111916. Epub 2023 Nov 7.

DOI:10.1016/j.plantsci.2023.111916

PMID:37944704

Abstract

DNA N-methyladenine (6 mA) is an evolutionarily conserved DNA modification in procaryotes and eukaryotes. The DNA 6 mA methylation is tightly controlled by 6 mA regulatory proteins. DNA N-adenine methyltransferase 1 (DAMT-1) has been identified as a DNA 6 mA methyltransferase in animals. In plants, DNA 6 mA methylation has been found, however, the DNA 6 mA methyltransferases and their function in plants are largely unknown. In our study, we find METTL4 is a DNA 6 mA methyltransferase in Arabidopsis thaliana. Both in vitro and in vivo evidences support the DNA 6 mA methyltransferase activity of METTL4. mettl4 mutant is hypersensitive to heat stress, suggesting DNA 6 mA methylation plays important role in heat stress adaption. RNA-seq and 6 mA IP-qPCR analysis show that METTL4 participates in heat stress tolerance by regulating expression of heat responsive genes. Our study find METTL4 is a plant DNA 6 mA methyltransferase and illustrates its function in regulating heat stress response.

摘要

DNA N-甲基腺嘌呤（6mA）是原核生物和真核生物中一种进化上保守的DNA修饰。DNA 6mA甲基化由6mA调节蛋白严格控制。DNA N-腺嘌呤甲基转移酶1（DAMT-1）已被鉴定为动物中的一种DNA 6mA甲基转移酶。在植物中，已发现DNA 6mA甲基化，然而，植物中的DNA 6mA甲基转移酶及其功能在很大程度上尚不清楚。在我们的研究中，我们发现METTL4是拟南芥中的一种DNA 6mA甲基转移酶。体外和体内证据均支持METTL4的DNA 6mA甲基转移酶活性。mettl4突变体对热胁迫高度敏感，表明DNA 6mA甲基化在热胁迫适应中起重要作用。RNA测序和6mA IP-qPCR分析表明，METTL4通过调节热响应基因的表达参与热胁迫耐受性。我们的研究发现METTL4是一种植物DNA 6mA甲基转移酶，并阐明了其在调节热胁迫反应中的功能。