Pharmaceutical Biotechnology Unit, Instituto Distrital de Ciencia, Biotecnología e Innovación en Salud, Bogotá 111611, Colombia.
Advanced Therapies Unit, Instituto Distrital de Ciencia, Biotecnología e Innovación en Salud, Bogotá 111611, Colombia.
Int J Mol Sci. 2023 Oct 24;24(21):15544. doi: 10.3390/ijms242115544.
Umbilical cord blood (UCB) serves as a source of hematopoietic stem and progenitor cells (HSPCs) utilized in the regeneration of hematopoietic and immune systems, forming a crucial part of the treatment for various benign and malignant hematological diseases. UCB has been utilized as an alternative HSPC source to bone marrow (BM). Although the use of UCB has extended transplantation access to many individuals, it still encounters significant challenges in selecting a histocompatible UCB unit with an adequate cell dose for a substantial proportion of adults with malignant hematological diseases. Consequently, recent research has focused on developing ex vivo expansion strategies for UCB HSPCs. Our results demonstrate that co-cultures with the investigated mesenchymal stromal cells (MSCs) enable a 10- to 15-fold increase in the cellular dose of UCB HSPCs while partially regulating the proliferation capacity when compared to HSPCs expanded with early acting cytokines. Furthermore, the secretory profile of UCB-derived MSCs closely resembles that of BM-derived MSCs. Moreover, both co-cultures exhibit alterations in cytokine secretion, which could potentially impact HSPC proliferation during the expansion process. This study underscores the fact that UCB-derived MSCs possess a remarkably similar supportive capacity to BM-derived MSCs, implying their potential use as feeder layers in the ex vivo expansion process of HSPCs.
脐带血（UCB）是造血干细胞和祖细胞（HSPCs）的来源，用于造血和免疫系统的再生，是治疗各种良性和恶性血液病的重要组成部分。UCB 已被用作骨髓（BM）的 HSPC 替代来源。尽管 UCB 的使用已经为许多人提供了移植的机会，但对于恶性血液病的大部分成年人来说，选择一个组织相容性好、细胞数量充足的 UCB 单位仍然面临着重大挑战。因此，最近的研究集中在开发 UCB HSPCs 的体外扩增策略上。我们的研究结果表明，与所研究的间充质基质细胞（MSCs）共培养可使 UCB HSPCs 的细胞数量增加 10-15 倍，同时与早期作用细胞因子扩增的 HSPCs相比，部分调节其增殖能力。此外，UCB 来源的 MSCs 的分泌谱与 BM 来源的 MSCs 非常相似。此外，两种共培养物均表现出细胞因子分泌的改变，这可能会在扩增过程中对 HSPC 增殖产生影响。这项研究强调了一个事实，即 UCB 来源的 MSCs 具有与 BM 来源的 MSCs 相似的支持能力，这意味着它们有可能作为 HSPC 体外扩增过程中的饲养层使用。
