用于监测溃疡性结肠炎疾病活动度变化的生物标志物。
Biomarkers for Monitoring of Changes in Disease Activity in Ulcerative Colitis.
作者信息
Tatsumi Yoshihiro, Kakimoto Kazuki, Hara Azusa, Mizuta Noboru, Numa Keijiro, Kinoshita Naohiko, Nakazawa Kei, Koshiba Ryoji, Hirata Yuki, Ota Kazuhiro, Miyazaki Takako, Nakamura Shiro, Sakagami Kayoko, Arimitsu Shoko, Ito Hiroaki, Nishikawa Hiroki
机构信息
2nd Department of Internal Medicine, Osaka Medical and Pharmaceutical University, Takatsuki City 569-8686, Japan.
Kinshukai Infusion Clinic, Osaka-shi 530-0011, Japan.
出版信息
J Clin Med. 2023 Nov 18;12(22):7165. doi: 10.3390/jcm12227165.
BACKGROUND
In recent years, various biomarkers of ulcerative colitis (UC) have emerged; however, few studies have simultaneously examined the utility of multiple biomarkers for monitoring disease activity. Additionally, serum leucine-rich alpha-2 glycoprotein (LRG), a new biomarker, may show a blunt response to anti-TNF antibody therapy. This prospective study explored effective biomarkers that could monitor disease activity changes in patients with UC. In addition, we examined the effect of anti-TNF antibody therapy on changes in LRG.
METHODS
Blood and stool samples were collected twice from patients with UC: at baseline and at least 8 weeks later. Changes in serum LRG, interleukin (IL)-6, prealbumin (pre-Alb), high-sensitivity C-reactive protein (hs-CRP), CRP, and fecal calprotectin (FC) were measured and correlated with changes in disease activity. The relationship between anti-TNF antibody therapy and LRG levels was also examined in patients with the same disease activity.
RESULTS
Forty-eight patients with UC (96 samples) were analyzed. ΔLRG and ΔIL-6 correlated strongly with the change in the partial Mayo (pMayo) score between the two time points (ΔpMayo) (r = 0.686, 0.635, respectively). In contrast, FC and IL-6 were particularly accurate predictors of clinical remission, and their area under the curves (AUCs) were significantly higher than that of CRP (AUC: 0.81, 0.76 vs. 0.50; = 0.001, 0.005). No association was found between the administration of anti-TNF antibody preparations and the LRG values.
CONCLUSIONS
Correlations were found between changes in UC disease activity and LRG, IL-6, pre-Alb, hs-CRP, CRP, and FC. LRG reflects disease activity during anti-TNF antibody therapy.
背景
近年来，溃疡性结肠炎（UC）的各种生物标志物不断涌现；然而，很少有研究同时检测多种生物标志物在监测疾病活动中的效用。此外，血清富含亮氨酸的α-2糖蛋白（LRG）作为一种新的生物标志物，可能对抗TNF抗体治疗反应迟钝。这项前瞻性研究探索了可监测UC患者疾病活动变化的有效生物标志物。此外，我们还研究了抗TNF抗体治疗对LRG变化的影响。
方法
从UC患者中采集两次血液和粪便样本：一次在基线时，另一次至少在8周后。检测血清LRG、白细胞介素（IL）-6、前白蛋白（pre-Alb）、高敏C反应蛋白（hs-CRP）、CRP和粪便钙卫蛋白（FC）的变化，并将其与疾病活动的变化相关联。在疾病活动相同的患者中，还研究了抗TNF抗体治疗与LRG水平之间的关系。
结果
对48例UC患者（96份样本）进行了分析。ΔLRG和ΔIL-6与两个时间点之间的部分梅奥（pMayo）评分变化（ΔpMayo）密切相关（r分别为0.686和0.635）。相比之下，FC和IL-6是临床缓解的特别准确的预测指标，它们的曲线下面积（AUC）显著高于CRP（AUC：0.81、0.76对0.50；P = 0.001、0.005）。未发现抗TNF抗体制剂的使用与LRG值之间存在关联。
结论
发现UC疾病活动变化与LRG、IL-6、pre-Alb、hs-CRP、CRP和FC之间存在相关性。LRG反映了抗TNF抗体治疗期间的疾病活动。
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