Department of Ophthalmology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Int J Mol Sci. 2023 Nov 20;24(22):16510. doi: 10.3390/ijms242216510.
Tear film instability is a major cause of dry eye disease. In order to treat patients with short tear film breakup time (TBUT)-type dry eye, the development of tear film stabilizing agents is essential. However, the lack of an appropriate animal model of tear film instability has made drug development difficult. Although rabbit dry eye models have been reported in the past, there are only a few reports that focus on tear film instability. Herein, we assessed the tear film stability of a rabbit dry eye model induced by dacryoadenectomy. A clinical evaluation of the ocular surface, interferometry, and histological assessments of the cornea and conjunctiva were performed. Following the removal of the lacrimal glands, TBUT was shortened significantly, with dimple and random breakup patterns prominently observed. Furthermore, the blink rate in this model increased after dacryoadenectomy, suggesting that this model partially captured the phenotypes of human short TBUT-type dry eye and may be useful as an animal model for investigating potential drug candidates.
泪膜不稳定是干眼症的主要原因。为了治疗泪膜破裂时间（TBUT）短型干眼症患者，开发泪膜稳定剂至关重要。然而，缺乏适当的泪膜不稳定动物模型使得药物开发变得困难。尽管过去有报道兔干眼症模型，但只有少数报道关注泪膜不稳定。在此，我们评估了通过泪腺切除术诱导的兔干眼症模型的泪膜稳定性。对眼表面进行临床评估、干涉测量以及角膜和结膜的组织学评估。在去除泪腺后，TBUT 明显缩短，明显观察到泪湖和随机破裂模式。此外，在泪腺切除术后，该模型的眨眼率增加，表明该模型部分捕获了人类短 TBUT 型干眼症的表型，并且可能作为研究潜在药物候选物的动物模型有用。
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