Kovačić Ines, Burić Petra, Žunec Ante, Bilić Josipa, Prgić Anamarija, Čanak Iva, Iveša Neven, Štifanić Mauro, Frece Jadranka
Faculty of Educational Sciences, Juraj Dobrila University of Pula, Zagrebačka 30, 52100 Pula, Croatia.
Faculty of Natural Sciences, Juraj Dobrila University of Pula, Zagrebačka 30, 52100 Pula, Croatia.
Microorganisms. 2023 Nov 7;11(11):2723. doi: 10.3390/microorganisms11112723.
The use of probiotics in the diet of bivalves poses a great potential in aquaculture as an alternative to antibiotics. The aim of this study was to assess the effect of I on the phenolic content and antioxidant capacity (AC) of queen scallop extracts after one month of feeding. Total phenols (TP) ranged from 28.17 ± 3.11 to 58.58 ± 8.57 mg GAE/100 g, total non-flavonoids (TNF) from 23.33 ± 3.66 to 36.56 ± 9.91 mg GAE/100 g, and total flavonoids (TF) from 10.56 ± 5.57 to 30.16 ± 1.69 mg CE/100 g. AC was assessed via three different methods: the ferric-reducing ability of plasma assay (FRAP), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic) acid assay (ABTS), and 2,2-diphenyl-1-picryhydrazyl assay (DPPH). FRAP values ranged from 0.13 ± 0.03 to 0.17 ± 0.02 µM AA/g, ABTS from 0.68 ± 0.11 to 2.79 ± 0.34 µM AA/g, and DPPH from 1.75 ± 0.17 to 2.98 ± 0.53 µM AA/g. Among all extracts, the best phenolic content and AC were observed in water extracts from queen scallops. The bivalves treated with the I-enriched diet showed higher AC according to the FRAP assay in all extracts. A significant correlation was observed between AC and TP and TNF in control and I-treated scallops.
在双壳贝类的饮食中使用益生菌作为抗生素的替代品,在水产养殖中具有巨大潜力。本研究的目的是评估喂食一个月后,[此处原文“I”指代不明,无法准确翻译]对皇后扇贝提取物中酚类含量和抗氧化能力(AC)的影响。总酚(TP)范围为28.17±3.11至58.58±8.57毫克没食子酸当量/100克,总非黄酮类(TNF)为23.33±3.66至36.56±9.91毫克没食子酸当量/100克,总黄酮类(TF)为10.56±5.57至30.16±1.69毫克儿茶素当量/100克。通过三种不同方法评估AC:血浆铁还原能力测定法(FRAP)、2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)酸测定法(ABTS)和2,2-二苯基-1-苦基肼测定法(DPPH)。FRAP值范围为0.13±0.03至0.17±0.02微摩尔抗坏血酸/克,ABTS为0.68±0.11至2.79±0.34微摩尔抗坏血酸/克,DPPH为1.75±0.17至2.98±0.53微摩尔抗坏血酸/克。在所有提取物中,皇后扇贝水提取物的酚类含量和AC最佳。根据FRAP测定法,用富含[此处原文“I”指代不明,无法准确翻译]的饲料处理的双壳贝类在所有提取物中均显示出较高的AC。在对照和经[此处原文“I”指代不明,无法准确翻译]处理的扇贝中,观察到AC与TP和TNF之间存在显著相关性。