Gu Yangyang, Ai Hongxu, Guo Tai, Liu Peng, Wang Yongqing, Zheng Hengbiao, Cheng Tao, Zhu Yan, Cao Weixing, Yao Xia
National Engineering and Technology Center for Information Agriculture (NETCIA), Zhongshan Biological Breeding Laboratory (ZSBBL), MARA Key Laboratory for Crop System Analysis and Decision Making, MOE Engineering Research Center of Smart Agriculture, Jiangsu Key Laboratory for Information Agriculture, Nanjing Agricultural University, One Weigang, Nanjing, Jiangsu, 210095, People's Republic of China.
Plant Methods. 2023 Nov 26;19(1):134. doi: 10.1186/s13007-023-01093-z.
The metrics for assessing the yield of crops in the field include the number of ears per unit area, the grain number per ear, and the thousand-grain weight. Typically, the ear number per unit area contributes the most to the yield. However, calculation of the ear number tends to rely on traditional manual counting, which is inefficient, labour intensive, inaccurate, and lacking in objectivity. In this study, two novel extraction algorithms for the estimation of the wheat ear number were developed based on the use of terrestrial laser scanning (TLS) in conjunction with the density-based spatial clustering (DBSC) algorithm based on the normal and the voxel-based regional growth (VBRG) algorithm. The DBSC involves two steps: (1) segmentation of the point clouds using differences in the normal vectors and (2) clustering of the segmented point clouds using a density clustering algorithm to calculate the ear number. The VBRG involves three steps: (1) voxelization of the point clouds, (2) construction of the topological relationships between the voxels as a connected region using the k-dimensional tree, and (3) detection of the wheat ears in the connected areas using a regional growth algorithm.
The results demonstrated that DBSC and VBRG were promising in estimating the number of ears for different cultivars, planting densities, N fertilization rates, and growth stages of wheat (RMSE = 76 ~ 114 ears/m, rRMSE = 18.62 ~ 27.96%, r = 0.76 ~ 0.84). Comparing the performance of the two algorithms, the overall accuracy of the DBSC (RMSE = 76 ears/m, rRMSE = 18.62%, r = 0.84) was better than that of the VBRG (RMSE = 114 ears/m, rRMSE = 27.96%, r = 0.76). It was found that with the DBSC, the calculation in points as units permitted more detailed information to be retained, and this method was more suitable for estimation of the wheat ear number in the field.
The algorithms adopted in this study provide new approaches for non-destructive measurement and efficient acquisition of the ear number in the assessment of the wheat yield phenotype.
评估田间作物产量的指标包括单位面积穗数、每穗粒数和千粒重。通常,单位面积穗数对产量的贡献最大。然而,穗数的计算往往依赖于传统的人工计数,这种方法效率低下、劳动强度大、不准确且缺乏客观性。在本研究中,基于地面激光扫描(TLS)结合基于法线的密度空间聚类(DBSC)算法和基于体素的区域生长(VBRG)算法,开发了两种用于估算小麦穗数的新型提取算法。DBSC算法包括两个步骤:(1)利用法线向量的差异对点云进行分割;(2)使用密度聚类算法对分割后的点云进行聚类以计算穗数。VBRG算法包括三个步骤:(1)点云的体素化;(2)使用k维树构建体素之间作为连通区域的拓扑关系;(3)使用区域生长算法在连通区域中检测小麦穗。
结果表明,DBSC和VBRG算法在估算不同品种、种植密度、氮肥施用量和小麦生长阶段的穗数方面具有潜力(均方根误差=76114穗/米,相对均方根误差=18.62%27.96%,相关系数r=0.76~0.84)。比较两种算法的性能,DBSC算法的总体精度(均方根误差=76穗/米,相对均方根误差=18.62%,相关系数r=0.84)优于VBRG算法(均方根误差=114穗/米,相对均方根误差=27.96%,相关系数r=0.76)。研究发现,使用DBSC算法,以点为单位进行计算能够保留更详细的信息,该方法更适合田间小麦穗数的估算。
本研究采用的算法为小麦产量表型评估中穗数的无损测量和高效获取提供了新方法。