Guo Rui, Wang Jingru, Zhao Wenshi, Cui Sicheng, Qian Sihan, Chen Qiuxu, Li Xue, Liu Yang, Zhang Qi
Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Changchun, 130103, China.
Key Laboratory of Functional Materials Physics and Chemistry of the Ministry of Education, Jilin Normal University, Changchun, 130103, China; Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Talanta. 2024 Mar 1;269:125466. doi: 10.1016/j.talanta.2023.125466. Epub 2023 Nov 23.
Human health is greatly threatened by bacterial infection, which raises the risk of serious illness and death in humans. For early screening and accurate treatment of bacterial infection, there is a strong desire to undertake ultrasensitive detection and effective killing of pathogenic bacteria. Herein, a novel surface-enhanced Raman scattering (SERS) biosensor based on sandwich structure consisting of capture probes/bacteria/SERS tags was established for specific identification, capture and photothermal killing of Escherichia coli (E. coli). Finite-difference time-domain (FDTD) technique was used to simulate the electromagnetic field distribution of capture probes, SERS tags and sandwich-type SERS substrate, and a possible SERS enhancement mechanism based on sandwich structure was presented and discussed. Sandwich-type SERS biosensor successfully achieved distinctive identification and magnetic beneficiation of E. coli. In addition, a single SERS substrate, including capture probes and SERS tags, could also achieve outstanding photothermal effects as a consequence of localized surface plasmon resonance (LSPR) effect. Intriguingly, sandwich-type SERS biosensor demonstrated a higher photothermal conversion efficiency (50.03 %) than the single substrate, which might be attributed to the formation of target bacterial clusters. The superior biocompatibility and the low toxicity of the sandwich-type biosensor were confirmed. Our approach offers a fresh method for constructing sandwich-type biosensor with multiple SERS hotspots based on extremely effective hybrid plasmonic nanoparticles, and has a wide range of potential applications in the recognition and treatment of bacteria.
细菌感染对人类健康构成了极大威胁，增加了人类患重病和死亡的风险。为了对细菌感染进行早期筛查和准确治疗，人们强烈希望能够对病原菌进行超灵敏检测并有效杀灭。在此，我们构建了一种基于捕获探针/细菌/SERS标签三明治结构的新型表面增强拉曼散射（SERS）生物传感器，用于特异性识别、捕获和光热杀灭大肠杆菌（E. coli）。采用时域有限差分（FDTD）技术模拟了捕获探针、SERS标签和三明治型SERS基底的电磁场分布，并提出和讨论了基于三明治结构的一种可能的SERS增强机制。三明治型SERS生物传感器成功实现了对大肠杆菌的独特识别和磁选。此外，由于局域表面等离子体共振（LSPR）效应，包含捕获探针和SERS标签的单一SERS基底也能实现出色的光热效应。有趣的是，三明治型SERS生物传感器表现出比单一基底更高的光热转换效率（50.03%），这可能归因于目标细菌簇的形成。证实了三明治型生物传感器具有优异的生物相容性和低毒性。我们的方法为基于极其有效的混合等离子体纳米粒子构建具有多个SERS热点的三明治型生物传感器提供了一种新方法，在细菌的识别和治疗方面具有广泛的潜在应用。
