Wuxi Fisheries College, Nanjing Agricultural University, Wuxi, 214081, PR China.
Jiangsu Haorun Biological Industry Group Co., Ltd, Taizhou, 225309, China.
Sci Data. 2023 Nov 30;10(1):843. doi: 10.1038/s41597-023-02761-4.
To gain a deeper understanding of the genetic factors influencing the growth and development of Eriocheir sinensis, a well-known species of hairy crab found in Yangcheng Lake, this study focused on the de novo genome and full-length transcriptome information of the selected subjects. Specifically, Yangcheng Lake hairy crabs were chosen as the experimental samples. Initially, a genome analysis was performed, resulting in the identification of gene fragments with a combined length of 1266,092,319 bp. Subsequently, a transcriptome analysis was conducted on a mixture of tissues from four different sites, namely muscle, brain, eye, and heart, to further investigate the genetic characteristics at the transcriptome level. The Pacific Biosciences (Pacio) single-molecule real-time sequencing system generated a total of 36.93 G sub-fragments and 175,90041 effective inserts. This research contributes to the indirect comprehension of genetic variations underlying individual traits. Furthermore, a comparison of the obtained data with relevant literature emphasizes the advantages of this study and establishes a basis for further investigations on the Chinese mitten crab.
为了更深入地了解影响中华绒螯蟹（Yangcheng Lake hairy crab）生长和发育的遗传因素，本研究专注于所选对象的从头基因组和全长转录组信息。具体来说，选择了阳澄湖大闸蟹作为实验样本。首先，进行了基因组分析，鉴定出了 1266092319bp 长度的基因片段。随后，对来自肌肉、大脑、眼睛和心脏四个不同部位的组织混合物进行了转录组分析，以进一步研究转录组水平的遗传特征。Pacific Biosciences（PacBio）单分子实时测序系统生成了总计 36.93G 的亚片段和 17590041 个有效插入片段。本研究有助于间接理解个体特征的遗传变异。此外，将获得的数据与相关文献进行比较，突出了本研究的优势，并为进一步研究中华绒螯蟹奠定了基础。
