UMR 152 PharmaDev, Université Paul Sabatier, Institut de Recherche pour le Développement.
UMR 152 PharmaDev, Université Paul Sabatier, Institut de Recherche pour le Développement;
J Vis Exp. 2023 Nov 17(201). doi: 10.3791/65877.
To study human gastrointestinal physiology, biomedical scientists have relied on the use of model organisms. Although many researchers have used mice as a model to study intestinal function, only a few reports have focused on Drosophila melanogaster (D. melanogaster). Compared to mice, fruit flies present many advantages, such as a short life cycle, cost-effective and simple maintenance, and no ethical issues. Furthermore, the mammalian gastrointestinal physiology, anatomy, and signaling pathways are highly conserved in D. melanogaster. Plant extracts have been used traditionally to treat diarrhea and constipation. For example, Psidium guajava (P. guajava) is one of the most known antidiarrheal agents in the tropics. However, no studies have evaluated the effect of antidiarrheal and laxative drugs and plant extracts in D. melanogaster, and it remains unknown if similar effects (e.g., smaller, more concentrated, and less abundant fecal deposits in the case of antidiarrheal drugs) can occur in the fruit flies compared to mammals. In this study, an antidiarrheal effect induced by P. guajava is demonstrated in a D. melanogaster strain that presents a diarrheic phenotype. Fecal sampling produced by flies is monitored using a dye-supplemented food. This protocol outlines the method used for preparing food with drugs, evaluating the fecal deposits of flies fed on these food preparations, and interpreting the data obtained.
为了研究人类胃肠道生理学，生物医学科学家依赖于使用模式生物。尽管许多研究人员已经使用小鼠作为模型来研究肠道功能，但只有少数报告集中在黑腹果蝇（Drosophila melanogaster，D. melanogaster）上。与小鼠相比，果蝇具有许多优点，例如生命周期短、成本效益高且易于维护，并且不存在伦理问题。此外，哺乳动物的胃肠道生理学、解剖结构和信号通路在 D. melanogaster 中高度保守。植物提取物在传统上被用于治疗腹泻和便秘。例如，番石榴（Psidium guajava，P. guajava）是热带地区最著名的止泻剂之一。然而，目前还没有研究评估抗腹泻和泻药药物以及植物提取物对 D. melanogaster 的影响，也不知道是否会在果蝇中出现类似的效果（例如，抗腹泻药物的粪便沉积物更小、更浓缩且更少）。在这项研究中，证明了番石榴在具有腹泻表型的 D. melanogaster 品系中诱导抗腹泻作用。使用添加染料的食物监测蝇产生的粪便取样。该方案概述了使用药物制备食物的方法、评估食用这些食物准备的蝇的粪便沉积物以及解释获得的数据。
