人源 smacovirus 1 Rep 域的晶体结构。

The crystal structure of the human smacovirus 1 Rep domain.

机构信息

Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.

出版信息

Acta Crystallogr F Struct Biol Commun. 2023 Dec 1;79(Pt 12):295-300. doi: 10.1107/S2053230X23009536. Epub 2023 Dec 5.

DOI:10.1107/S2053230X23009536

PMID:38051309

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10833120/

Abstract

Replication initiator proteins (Reps) from the HUH endonuclease family process specific single-stranded DNA sequences to initiate rolling-circle replication in viruses. Here, the first crystal structure of the apo state of a Rep domain from the smacovirus family is reported. The structure of the human smacovirus 1 Rep domain was obtained at 1.33 Å resolution and represents an expansion of the HUH endonuclease superfamily, allowing greater diversity in bioconjugation-tag applications.

摘要

复制起始蛋白 (Reps) 来自 HUH 内切酶家族，它们可以处理特定的单链 DNA 序列，从而在病毒中启动滚环复制。本文报道了 smacovirus 家族 Rep 结构域的 apo 状态的首个晶体结构。人源 smacovirus 1 Rep 结构域的晶体结构分辨率为 1.33 Å，属于 HUH 内切酶超家族的扩展，为生物偶联标签的应用提供了更大的多样性。