Department of Neurology, the First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region, 530021, China.
State Key Laboratory of Targeting Oncology, National Center for International Research of Biotargeting Theranostics, Guangxi Key Laboratory of Biotargeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University, Nanning 530021, China.
Math Biosci Eng. 2023 Oct 10;20(10):18939-18959. doi: 10.3934/mbe.2023838.
Immune infiltration plays a pivotal role in the pathogenesis of ischemic stroke. A novel form of cell death known as disulfidptosis has emerged in recent studies. However, there is currently a lack of research investigating the regulatory mechanism of disulfidptosis-related genes in immune infiltration during ischemic stroke. Using machine learning methods, we identified candidate key disulfidptosis-related genes (DRGs). Subsequently, we performed an analysis of immune cell infiltration to investigate the dysregulation of immune cells in the context of ischemic stroke. We assessed their diagnostic value by employing receiver operating characteristic (ROC) curves. To gain further insights, we conducted functional enrichment analyses to elucidate the signaling pathways associated with these seven DRGs. We identified two distinct subclusters based on the expression patterns of these seven DRGs. The unique roles of these subclusters were further evaluated through KEGG analysis and immune infiltration studies. Furthermore, we validated the expression profiles of these seven DRGs using both single-cell datasets and external datasets. Lastly, molecular docking was performed to explore potential drugs for the treatment of ischemic stroke. We identified seven DRGs. The seven DRGs are related to immune cells. Additionally, these seven DRGs also demonstrate potential diagnostic value in ischemic stroke. Functional enrichment analysis highlighted pathways such as platelet aggregation and platelet activation. Two subclusters related to disulfidptosis were defined, and functional enrichment analysis of their differentially expressed genes (DEGs) primarily involved pathways like cytokine-cytokine receptor interaction. Single-cell analysis indicated that these seven DRGs were primarily distributed among immune cell types. Molecular docking results suggested that genistein might be a potential therapeutic drug. This study has opened up new avenues for exploring the causes of ischemic stroke and developing potential therapeutic targets.
免疫浸润在缺血性中风的发病机制中起着关键作用。最近的研究中出现了一种新的细胞死亡形式，称为二硫键细胞死亡。然而，目前还没有研究探讨二硫键相关基因在缺血性中风免疫浸润中的调控机制。我们使用机器学习方法，确定了候选关键二硫键相关基因（DRGs）。随后，我们对免疫细胞浸润进行了分析，以研究缺血性中风背景下免疫细胞的失调。我们通过接收者操作特征（ROC）曲线评估了它们的诊断价值。为了进一步深入了解，我们进行了功能富集分析，以阐明与这七个 DRGs 相关的信号通路。我们根据这七个 DRGs 的表达模式确定了两个不同的亚群。通过 KEGG 分析和免疫浸润研究进一步评估了这些亚群的独特作用。此外，我们使用单细胞数据集和外部数据集验证了这七个 DRGs 的表达谱。最后，我们进行了分子对接，以探索治疗缺血性中风的潜在药物。我们确定了七个 DRGs。这七个 DRGs 与免疫细胞有关。此外，这七个 DRGs 在缺血性中风中也具有潜在的诊断价值。功能富集分析强调了血小板聚集和血小板激活等途径。定义了两个与二硫键细胞死亡相关的亚群，其差异表达基因（DEGs）的功能富集分析主要涉及细胞因子-细胞因子受体相互作用等途径。单细胞分析表明，这七个 DRGs 主要分布在免疫细胞类型中。分子对接结果表明，染料木黄酮可能是一种有潜力的治疗药物。这项研究为探索缺血性中风的病因和开发潜在的治疗靶点开辟了新的途径。
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