Division of Nutrition, St. John's Research Institute, St. John's National Academy of Health Sciences, Bangalore, India.
Division of Nutrition, St. John's Research Institute, St. John's National Academy of Health Sciences, Bangalore, India.
Free Radic Biol Med. 2024 Jan;210:344-351. doi: 10.1016/j.freeradbiomed.2023.11.042. Epub 2023 Dec 4.
Type 2 Diabetes (T2DM) has been linked to ferroptosis. This study aimed to assess expression levels of genes linked with iron metabolism in peripheral blood mononuclear cells (PBMCs) from T2DM patients and to investigate the association of these expression levels with anthropometric and clinical parameters.
Gene expression of iron metabolism genes (Ferritin Light Chain, FTL; Ferritin Heavy Chain, FTH1; Transferrin Receptor, TFRC; Divalent Metal Transporter 1, SLC11A2; Ferroportin, SLC40A1) in archival PBMCs was assessed using quantitative real-time PCR assays. Correlations of gene expression with anthropometric/biochemical patient data were evaluated.
The study included 36 (18 male/18 female) T2DM patients and 45 (28 male/17 female) normoglycemic (NGT) subjects with a mean age of 38.1 ± 6.8 years and 47.6 ± 8.6 years respectively. Relative expression of FTL was significantly lower in T2DM females compared to that in NGT females (P = 0.027). Relative expression of SLC40A1 was significantly lower in the T2DM group (P = 0.043) and in the T2DM females (P = 0.021). Relative expression of SLC11A2 was negatively correlated with systolic blood pressure in T2DM male patients. Relative expression of SLC40A1 was negatively associated with serum phosphorous and positively associated with serum thyroid stimulating hormone in male T2DM patients.
Our findings indicate a reduction in the expression of FTL in perimenopausal T2DM females. Also, in male T2DM patients and NGT subjects, biochemical markers are significantly correlated with the expression of FTL, FTH1, SLC11A2, and SLC40A1 in PBMCs.
2 型糖尿病（T2DM）与铁死亡有关。本研究旨在评估 T2DM 患者外周血单个核细胞（PBMC）中与铁代谢相关的基因表达水平，并探讨这些表达水平与人体测量学和临床参数的相关性。
使用定量实时 PCR 检测铁代谢基因（铁蛋白轻链，FTL；铁蛋白重链，FTH1；转铁蛋白受体，TFRC；二价金属转运蛋白 1，SLC11A2；亚铁转运蛋白，SLC40A1）在存档 PBMC 中的基因表达。评估基因表达与人体测量学/生化患者数据的相关性。
本研究纳入了 36 名（18 名男性/18 名女性）T2DM 患者和 45 名（28 名男性/17 名女性）血糖正常（NGT）受试者，平均年龄分别为 38.1±6.8 岁和 47.6±8.6 岁。与 NGT 女性相比，T2DM 女性 FTL 的相对表达明显降低（P=0.027）。T2DM 组（P=0.043）和 T2DM 女性（P=0.021）的 SLC40A1 相对表达明显降低。T2DM 男性患者中 SLC11A2 的相对表达与收缩压呈负相关。T2DM 男性患者中 SLC40A1 的相对表达与血清磷呈负相关，与血清促甲状腺激素呈正相关。
本研究结果表明，围绝经期 T2DM 女性 FTL 的表达减少。此外，在 T2DM 男性患者和 NGT 受试者中，生化标志物与 PBMC 中 FTL、FTH1、SLC11A2 和 SLC40A1 的表达呈显著相关。
