一种具有集成原位成像和 pH 检测的细胞状态监测系统。

A Cell State Monitoring System with Integrated In Situ Imaging and pH Detection.

机构信息

State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.

Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Sensors (Basel). 2023 Nov 22;23(23):9340. doi: 10.3390/s23239340.

DOI:10.3390/s23239340

PMID:38067713

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10708649/

Abstract

Cell models are one of the most widely used basic models in biological research, and a variety of in vitro cell culture techniques and models have been developed recently to simulate the physiological microenvironment in vivo. However, regardless of the technique or model, cell culture is the most fundamental but crucial component. As a result, we have developed a cell culture monitoring system to assess the functional status of cells within a biochip. This article focuses on a mini-microscope made from a readily available camera for in situ continuous observation of cell growth within a biochip and a pH sensor based on optoelectronic sensing for measuring pH. With the aid of this monitoring system, scientists can keep an eye on cell growth in real time and learn how the pH of the culture medium affects it. This study offers a new approach for tracking cells on biochips and serves as a valuable resource for enhancing cell culture conditions.

摘要

细胞模型是生物研究中最广泛使用的基础模型之一，最近已经开发出各种体外细胞培养技术和模型来模拟体内生理微环境。然而，无论使用哪种技术或模型，细胞培养都是最基础但也是最关键的组成部分。因此，我们开发了一种细胞培养监测系统，以评估生物芯片内细胞的功能状态。本文重点介绍了一种由现成相机制成的微型显微镜，用于对生物芯片内细胞生长进行原位连续观察，以及一种基于光电传感的 pH 传感器，用于测量 pH 值。借助这个监测系统，科学家们可以实时观察细胞的生长情况，并了解培养基的 pH 值如何影响细胞的生长。这项研究为在生物芯片上跟踪细胞提供了一种新方法，为改善细胞培养条件提供了有价值的资源。