利用姜黄素的光动力特性破坏痤疮丙酸杆菌生物膜：一种治疗痤疮的有前景的方法。

Utilizing the photodynamic properties of curcumin to disrupt biofilms in Cutibacterium acnes: A promising approach for treating acne.

作者信息

Zheng Nana, Xie Yuanyuan, Zhou Meng, Liu Yuzhen, Xu Haoxiang, Zeng Rong, Wan Chunping, Li Min

机构信息

Department of Dermatology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210003, China.

Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Institute of Dermatology, Center for Global Health, School of Public Health, Nanjing Medical University, Hospital for Skin Diseases, Chinese Academy of Medical Sciences & Peking Union Medical College, 12 Jiang Wang Miao Street, Nanjing, Jiangsu 210042, China.

出版信息

Photodiagnosis Photodyn Ther. 2024 Feb;45:103928. doi: 10.1016/j.pdpdt.2023.103928. Epub 2023 Dec 7.

DOI:10.1016/j.pdpdt.2023.103928

PMID:38070633

Abstract

BACKGROUND

The treatment of acne vulgaris is often challenging due to the antibiotic resistance frequently observed in Cutibacterium acnes (C.acnes), a prevalent bacterium linked to this condition.

OBJECTIVE

The objective of this research was to examine the impact of curcumin photodynamic therapy (PDT) on the survival of C.acnes and activity of biofilms produced by this microorganism.

METHODS

Following the Clinical and Laboratory Standards Institute (CLSI) guidelines, we assessed the drug sensitivity of 25 clinical C.acnes strains to five antibiotics (erythromycin, clindamycin, tetracycline, doxycycline, minocycline) and curcumin by implementing the broth microdilution technique. In addition, we established C.acnes biofilms in a laboratory setting and subjected them to curcumin-PDT(curcumin combined with blue light of 180 J/cm). Afterwards, we evaluated their viability using the XTT assay and observed them using confocal laser scanning microscopy.

RESULTS

The result revealed varying resistance rates among the tested antibiotics and curcumin, with erythromycin, clindamycin, tetracycline, doxycycline, minocycline, and curcumin exhibiting resistance rates of 72 %, 44 %, 36 %, 28 %, 0 %, and 100 %, respectively. In the curcumin-PDT inhibition tests against four representative antibiotic-resistant strains, it was found that the survival rate of all strains of planktonic C. acnes was reduced, and the higher the concentration of curcumin, the lower the survival rate. Furthermore, in the biofilm inhibition tests, the vitality and three-dimensional structure of the biofilms were disrupted, and the inhibitory effect became more significant with higher concentrations of curcumin.

CONCLUSION

The results emphasize the possibility of using curcumin PDT as an alternative approach for the treatment of C.acnes, especially in instances of antibiotic-resistant variations and infections related to biofilms.

摘要

背景

寻常痤疮的治疗通常具有挑战性，因为在痤疮丙酸杆菌（C.acnes）中经常观察到抗生素耐药性，痤疮丙酸杆菌是一种与这种疾病相关的常见细菌。

目的

本研究的目的是检验姜黄素光动力疗法（PDT）对痤疮丙酸杆菌存活及该微生物产生的生物膜活性的影响。

方法

按照临床和实验室标准协会（CLSI）指南，我们通过肉汤微量稀释技术评估了25株临床痤疮丙酸杆菌菌株对五种抗生素（红霉素、克林霉素、四环素、多西环素、米诺环素）和姜黄素的药物敏感性。此外，我们在实验室环境中建立了痤疮丙酸杆菌生物膜，并对其进行姜黄素 - PDT（姜黄素与180 J/cm的蓝光联合使用）处理。之后，我们使用XTT法评估其活力，并使用共聚焦激光扫描显微镜进行观察。

结果

结果显示，受试抗生素和姜黄素的耐药率各不相同，红霉素、克林霉素、四环素、多西环素、米诺环素和姜黄素的耐药率分别为72%、44%、36%、28%、0%和100%。在针对四种代表性耐药菌株的姜黄素 - PDT抑制试验中，发现所有浮游痤疮丙酸杆菌菌株的存活率均降低，且姜黄素浓度越高，存活率越低。此外，在生物膜抑制试验中，生物膜的活力和三维结构被破坏，姜黄素浓度越高，抑制作用越显著。