Assessment of Antimicrobial Activity and Cytotoxic Effect of Nigella sativa, Syzygium aromaticum, and Allium cepa Formulation for Use As Antimicrobial Gel or Mouthwash.

BACKGROUND

Black cumin/ is traditionally used for its wide range of medicinal effects. Clove/ is well known as a natural antibiotic with antiviral, antibacterial, and antifungal properties, and onion/ for its antibacterial properties. The combination of these extracts can effectively have many therapeutic applications.

AIM

The aim of the present study is to prepare the formulation of  and to assess the antimicrobial activity and cytotoxic effect of the preparedformulation.

MATERIALS AND METHODS

Antimicrobial activity was carried out by agar well diffusion method and cytotoxic activity was carried out using the brine shrimp lethality assay method.

RESULTS

The antimicrobial activity showed the zone of inhibition was highest with  showing 22 mm of zone of inhibition at 100 µl. The cytotoxicity test showed there are about 90% live nauplii in the lowest concentration at 5 µl.

CONCLUSION

The study concluded that the combination extract of  could be used as an antimicrobial agent. Further research with pathogenic oral microorganisms can validate this formulation as an antimicrobial gel or mouthwash for various pathologies.