Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, Bellaterra, Catalonia 08193, Spain.
Environ Sci Technol. 2023 Dec 26;57(51):21727-21735. doi: 10.1021/acs.est.3c05455. Epub 2023 Dec 11.
The EU low-carbon economy aims to reduce the level of CO emission in the EU to 80% by 2050. High efforts are required to achieve this goal, where successful CCU (Carbon Capture and Utilization) technologies will have a high impact. Biocatalysts offer a greener alternative to chemical catalysts for the development of CCU strategies since biocatalysis conforms 10 of the 12 principles of green chemistry. In this study, a multienzymatic system, based on alcohol dehydrogenase (ADH), pyruvate decarboxylase (PDC), and lactate dehydrogenase (LDH), that converts CO and ethanol into lactic acid leading to a 100% atom economy was studied. The system allows cofactor regeneration, thus reducing the process cost. Through reaction media engineering and enzyme ratio study, the performance of the system was able to produce up to 250 μM of lactic acid under the best conditions using 100% CO, corresponding to the highest concentration of lactic acid obtained up to date using this multienzymatic approach. For the first time, the feasibility of the system to be applied under a real industrial environment has been tested using synthetic gas mimicking real blast furnace off-gases composition from the iron and steel industry. Under these conditions, the system was also capable of producing lactic acid, reaching 62 μM.
欧盟低碳经济的目标是到 2050 年将欧盟的 CO 排放量水平降低 80%。要实现这一目标需要付出巨大的努力，而成功的 CCU（碳捕获与利用）技术将产生重大影响。生物催化剂为 CCU 策略的发展提供了比化学催化剂更环保的选择，因为生物催化符合绿色化学的 12 条原则中的 10 条。在这项研究中，研究了一种基于醇脱氢酶 (ADH)、丙酮酸脱羧酶 (PDC) 和乳酸脱氢酶 (LDH) 的多酶系统，该系统将 CO 和乙醇转化为乳酸，实现了 100%的原子经济性。该系统允许辅酶再生，从而降低了工艺成本。通过反应介质工程和酶比例研究，在最佳条件下，该系统能够使用 100%的 CO 生产高达 250 μM 的乳酸，这是迄今为止使用这种多酶方法获得的乳酸最高浓度。该系统首次被测试应用于真实工业环境的可行性，使用模拟钢铁行业高炉废气成分的合成气。在这些条件下，该系统也能够生产乳酸，达到 62 μM。
