[Hydatid cyst sampling by FTA Card method for characterizing sensu lato in Algeria. Preliminary results].

INTRODUCTION AND OBJECTIVES

Cystic echinococcosis is highly endemic in Algeria and constitutes a major socio-economic problem. Typing the species of the sensu lato complex circulating in cattle requires the use of a hydatid cyst sampling method adapted to difficult field conditions (high heat and humidity, long transport time). The FTA Card method currently constitutes an effective means of preserving biological samples before their molecular analysis. In the present study, the FTA Card method was used in the collection of hydatid cysts to identify the species of sensu lato circulating in ruminants (intermediate hosts) in eastern Algeria.

MATERIAL AND METHODS

A PCR was carried out for 41 samples of hydatid cysts taken from six slaughterhouses in eastern Algeria, targeting the mitochondrial gene. PCR products were visualized by electrophoresis in a 1% agarose gel.

RESULTS AND CONCLUSION

The results of the molecular analysis of all hydatid cyst samples confirmed the presence of sensu stricto in sheep, cattle and camels. The ubiquitous nature of the G1 genotype has been demonstrated. The use of FTA Card sampling is an efficient and simple method to obtain a biological sample in order to characterize the species of sensu lato in Algeria. The good preservation of the DNA in this matrix will make it easier to obtain new molecular data from difficult regions. The identification of the species of the sensu lato complex involved in the biological cycle is an essential prerequisite for the implementation of control measures, since different host species participate in their evolutionary cycle. The characterization of genotypes is essential to define an appropriate control strategy against cystic echinococcosis.