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[不同人类外周血保存及研究准备方法对红细胞形态的影响(扫描电子显微镜观察)]

[Effect of various methods of the preservation and preparation for study of human peripheral blood on erythrocyte morphology (scanning electron microscopy)].

作者信息

Mechenov G, Dimitrov M

出版信息

Eksp Med Morfol. 1979;18(2):106-12.

PMID:380965
Abstract

In the paper the authors describe the influence of various ways of preservation and preparation of peripheral human blood on the morphology of erythrocytes. The investigation was performed by a scanning electrone microscope JSM-35 JEOL--Japan). The basic types of erythrocytes were shown, which were encountered in the samples. It was established that dehydratation and various methods for fixation of erythrocytes did not affect their morphology. The authors found changes, which occurred in erythrocyte morphology after blood preservation in anticoagulant,FBFR and as a whole blood at various intervals. The action of centrifugal forces in the process of centrifugation of blood on various types of erythocytes was indicated as well. The results of the studies were illustrated with 21 pictures, made at magnification of 3000 X up to 15 000 X.

摘要

在该论文中,作者描述了外周血的各种保存和制备方式对红细胞形态的影响。研究采用日本电子光学实验室(JEOL)的JSM - 35扫描电子显微镜进行。展示了样本中出现的红细胞基本类型。确定红细胞脱水和各种固定方法不影响其形态。作者发现了血液在抗凝剂、FBFR中以及全血状态下不同时间保存后红细胞形态发生的变化。还指出了血液离心过程中离心力对不同类型红细胞的作用。研究结果用21张放大倍数为3000倍至15000倍的图片进行了说明。

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