转录组分析揭示 gE/gI/TK 缺失对 PRV 感染宿主反应的影响。
Transcriptomic analysis reveals impact of gE/gI/TK deletions on host response to PRV infection.
机构信息
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Taian, China.
Shandong Collaborative Innovation Center for Development of Veterinary Pharmaceuticals, College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
出版信息
Virol J. 2023 Dec 19;20(1):303. doi: 10.1186/s12985-023-02265-y.
BACKGROUND
Pseudorabies virus (PRV) causes substantial losses in the swine industry worldwide. Attenuated PRV strains with deletions of immunomodulatory genes glycoprotein E (gE), glycoprotein I (gI) and thymidine kinase (TK) are candidate vaccines. However, the effects of gE/gI/TK deletions on PRV-host interactions are not well understood.
METHODS
To characterize the impact of gE/gI/TK deletions on host cells, we analyzed and compared the transcriptomes of PK15 cells infected with wild-type PRV (SD2017), PRV with gE/gI/TK deletions (SD2017gE/gI/TK) using RNA-sequencing.
RESULTS
The attenuated SD2017gE/gI/TK strain showed increased expression of inflammatory cytokines and pathways related to immunity compared to wild-type PRV. Cell cycle regulation and metabolic pathways were also perturbed.
CONCLUSIONS
Deletion of immunomodulatory genes altered PRV interactions with host cells and immune responses. This study provides insights into PRV vaccine design.
背景
伪狂犬病病毒（PRV）在全球范围内给养猪业造成了巨大损失。缺失免疫调节基因糖蛋白 E（gE）、糖蛋白 I（gI）和胸苷激酶（TK）的减毒 PRV 株是候选疫苗。然而，gE/gI/TK 缺失对 PRV-宿主相互作用的影响尚不清楚。
方法
为了研究 gE/gI/TK 缺失对宿主细胞的影响，我们使用 RNA 测序分析和比较了野生型 PRV（SD2017）和缺失 gE/gI/TK 的 PRV（SD2017gE/gI/TK）感染 PK15 细胞的转录组。
结果
与野生型 PRV 相比，减毒的 SD2017gE/gI/TK 株表现出炎症细胞因子和与免疫相关的途径表达增加。细胞周期调控和代谢途径也受到干扰。
结论
免疫调节基因的缺失改变了 PRV 与宿主细胞和免疫反应的相互作用。本研究为 PRV 疫苗设计提供了思路。
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