癌症易感性 18 通过海绵吸附 miR-5586-5p 正向调节 NUAK 家族激酶 1 的表达，促进宫颈癌细胞的迁移和侵袭。
Cancer susceptibility 18 positively regulates NUAK Family Kinase 1 expression to promote migration and invasion via sponging of miR-5586-5p in cervical cancer cells.
机构信息
Translational Medicine Centre, Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.
Hunan Provincial Clinical Research Centre for Oncoplastic Surgery, Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.
出版信息
Int J Immunopathol Pharmacol. 2023 Jan-Dec;37:3946320231223310. doi: 10.1177/03946320231223310.
INTRODUCTION
Cervical squamous cell carcinoma (CESC) is the most common gynecological malignancy worldwide. Although the gene was involved in the regulation of cancer biology, its specific role in CESC is not well characterized.
METHODS
-related axis was predicted by bioinformatic analyses, and the competing endogenous RNA (ceRNA) interaction was further validated using quantitative real-time PCR, western blotting, RNA pulldown, and luciferase reporter assays. Transwell and wound healing assays were performed to verify the effect of on SiHa and HeLa cell motility.
RESULTS
We found that was upregulated in CESC tissues. Moreover, interference with attenuated -mediated epithelial-mesenchymal transition (EMT) and thus suppressed cancer cell motility. Furthermore, the effects of knockdown on CESC cells were partly rescued by transfection with the miR-5586-5p inhibitor. Additionally, our findings indicated that acts as a ceRNA to enhance expression by sponging miR-5586-5p.
CONCLUSION
Our study showed a novel /miR-5586-5p/ ceRNA axis that could regulate cell invasion and migration by modulating EMT in CESC. These findings suggest that may potentially serve as a novel therapeutic target in CESC treatment.
简介
子宫颈鳞状细胞癌（CESC）是全球最常见的妇科恶性肿瘤。虽然 基因参与了癌症生物学的调控，但它在 CESC 中的具体作用尚未得到很好的描述。
方法
通过生物信息学分析预测 相关轴，并通过定量实时 PCR、western blot、RNA 下拉和荧光素酶报告基因测定进一步验证竞争内源性 RNA（ceRNA）相互作用。进行 Transwell 和划痕愈合实验以验证 对 SiHa 和 HeLa 细胞迁移的影响。
结果
我们发现 在上皮性卵巢癌组织中上调。此外，干扰 减弱了 介导的上皮间质转化（EMT），从而抑制了癌细胞的迁移。此外，miR-5586-5p 抑制剂转染部分挽救了 敲低对 CESC 细胞的影响。此外，我们的研究结果表明， 作为 ceRNA 通过海绵吸附 miR-5586-5p 来增强 表达。
结论
我们的研究表明，在 CESC 中，通过调节 EMT，可以调控细胞侵袭和迁移的 /miR-5586-5p/ ceRNA 轴。这些发现表明， 可能成为 CESC 治疗的新的治疗靶点。
Zotero 插件