Lotsios Nikolaos S, Arvanitis Nikolaos, Charonitakis Alexandros G, Mpekoulis George, Frakolaki Efseveia, Vassilaki Niki, Sideris Diamantis C, Vassilacopoulou Dido
Section of Biochemistry and Molecular Biology, Department of Biology, National and Kapodistrian University of Athens, 15701 Athens, Greece.
Laboratory of Molecular Virology, Hellenic Pasteur Institute (HPI), 11521 Athens, Greece.
Curr Issues Mol Biol. 2023 Dec 16;45(12):10179-10192. doi: 10.3390/cimb45120635.
Oxidative stress is known to influence mRNA levels, translation, and proteolysis. The importance of oxidative stress has been demonstrated in several human diseases, including neurodegenerative disorders. L-Dopa decarboxylase (DDC) is the enzyme that converts L-Dopa to dopamine (DA). In spite of a large number of studies, little is known about the biological significance of the enzyme under physiological and pathological conditions. Here, we investigated the relationship between DDC expression and oxidative stress in human neural and non-neural cells. Oxidative stress was induced by treatment with HO. Our data indicated that mRNA and protein expression of DDC was enhanced or remained stable under conditions of ROS induction, despite degradation of total RNA and increased cytotoxicity and apoptosis. Moreover, DDC silencing caused an increase in the HO-induced cytotoxicity. The current study suggests that DDC is involved in the mechanisms of oxidative stress.
已知氧化应激会影响信使核糖核酸（mRNA）水平、翻译和蛋白水解。氧化应激的重要性已在包括神经退行性疾病在内的多种人类疾病中得到证实。左旋多巴脱羧酶（DDC）是一种将左旋多巴转化为多巴胺（DA）的酶。尽管进行了大量研究，但对于该酶在生理和病理条件下的生物学意义仍知之甚少。在此，我们研究了人类神经细胞和非神经细胞中DDC表达与氧化应激之间的关系。通过用HO处理诱导氧化应激。我们的数据表明，尽管总RNA降解、细胞毒性增加和细胞凋亡增加，但在活性氧（ROS）诱导条件下，DDC的mRNA和蛋白表达增强或保持稳定。此外，DDC沉默导致HO诱导的细胞毒性增加。当前研究表明DDC参与氧化应激机制。
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