Cui Baolu, Yu Min, Bai Jiaojiao, Zhu Zhiguo
College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang 332005, China.
College of Biological Sciences and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China.
Metabolites. 2023 Dec 11;13(12):1195. doi: 10.3390/metabo13121195.
Hypertrophy development induced by the overexpression of (also called ) was susceptible to in tomatoes. Transcriptome and metabolome analyses were performed on the hypertrophy leaves of a -overexpressed line (OE) and wild type (WT) to investigate the molecular mechanism. Metabolome analysis revealed that six key metabolites were over-accumulated in the OE, including Acetylserine/O-Acetyl-L-serine, Glucono-1,5-lactone, Gluconate, 2-Oxoglutarate, and Loganate, implying that the OE plants increased salt or oxidant resistance under normal growth conditions. The RNA-seq analysis showed the changed expressions of downstream genes involved in high-energy consumption, photosynthesis, and transcription regulation in OE lines, and we hypothesized that these biological processes were related to the GTgamma subfamily of trihelix factors. The RT-PCR results showed that the expressions of the genes in tomatoes, i.e., - and -, were suppressed in the hypertrophy development. The expression of the gene was downregulated by salinity, indicating a coordinated role of GTgamma in hypertrophy development and salt stress. Further research showed that both - and - were highly expressed in leaves and could be significantly induced by abscisic acid (ABA). The GTgamma protein had a putative phosphorylation site at S. These results suggested GTgamma's role in hypertrophy development by increasing the salt resistance.
在番茄中，由（也称为）过表达诱导的肥大发育对[具体物质或因素未明确给出]敏感。对过表达系（OE）和野生型（WT）的肥大叶片进行了转录组和代谢组分析，以研究其分子机制。代谢组分析表明，在OE中六种关键代谢物过度积累，包括乙酰丝氨酸/O - 乙酰 - L - 丝氨酸、葡萄糖酸 - 1,5 - 内酯、葡萄糖酸盐、2 - 酮戊二酸和马钱子酸，这意味着OE植株在正常生长条件下增强了耐盐性或抗氧化性。RNA测序分析显示，OE系中参与高能量消耗、光合作用和转录调控的下游基因表达发生了变化，我们推测这些生物学过程与三螺旋因子的GTgamma亚家族有关。逆转录 - PCR结果表明，番茄中[具体基因未明确给出]基因，即[基因名称未明确给出] - 和[基因名称未明确给出] - ，在肥大发育过程中表达受到抑制。[具体基因未明确给出]基因的表达受到盐度下调，表明GTgamma在肥大发育和盐胁迫中具有协同作用。进一步研究表明，[具体基因未明确给出] - 和[具体基因未明确给出] - 在叶片中高表达，并且可以被脱落酸（ABA）显著诱导。GTgamma蛋白在S处有一个推定的磷酸化位点。这些结果表明GTgamma通过增强耐盐性在肥大发育中发挥作用。
