Hypoxia induced cell dormancy of salivary adenoid cystic carcinoma through miR-922/DEC2 axis.

作者信息

Dai Li, Xian Hongchun, Wang Haofan, Li Mao, Zhang Mei, Liang Xin-Hua, Tang Ya-Ling

机构信息

State Key Laboratory of Oral Diseases and National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, China; Shandong Provincial Hospital Affiliated to Shandong First Medical University, China.

出版信息

Transl Oncol. 2024 Feb;40:101868. doi: 10.1016/j.tranon.2023.101868. Epub 2023 Dec 22.

DOI:10.1016/j.tranon.2023.101868

PMID:38141378

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10751830/

Abstract

BACKGROUND

Hypoxia has been shown to induce cancer cells to become dormant meanwhile these cells inclined to disseminate and eventually cause metastasis. However, the molecular mechanism is still elusive. The purpose is to explore whether dormancy-associated microRNAs (DmiRs) get involved in hypoxia-induced cell dormancy of salivary adenoid cystic carcinoma (SACC).

MATERIAL AND METHODS

This study performed multi-perspective investigation of the biological effects of miR-922/DEC2 on SACC based on clinical samples, 2D and 3D in vitro model and nude mice in vivo model, based on our previous study of overexpression of DEC2 inducing SACC cellular dormancy.

RESULTS

According to the existing microRNA array of SACC tissue, we found that miR-922 was upregulated in SACC tissue and was inversely correlated with DEC2, suggesting that miR-922 might participate in the activation of SACC cell dormancy as a DmiR. Then, we found miR-922 SACC cells exhibited cell dormancy and a low level of fatty acid oxidation with propensity for lipid droplets accumulation through DEC2. Moreover, HIF1a downregulated the level of miR-922 to induce SACC cell dormancy. In addition, in xenografts of nude mice the inhibition of miR-922 attenuated the growth of primary tumor and the lung metastasis of SACC.