Pharmacological and Diagnostic Research Center, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, 19328, Jordan.
College of Pharmacy, Ras Al Khaimah Medical and Health Sciences University, Ras Al Khaimah, United Arab Emirates.
Chem Biodivers. 2024 Feb;21(2):e202301470. doi: 10.1002/cbdv.202301470. Epub 2024 Jan 18.
Doxorubicin (DOX) is widely used against solid tumors. Niosomes are self-assembled nanocarriers of non-ionic surfactants. DOX loaded into cationic niosomes (DOX-Nio) was prepared via thin film hydration method. DOX-Nio was then decorated with a hyaluronic acid (DOX-HA-Nio) via electrostatic interaction. DOX-Nio and DOX-HA-Nio displayed a particle size of 120.0±1.02 and 182.9±2.3 nm, and charge of + 35.5±0.15 and -15.6±0.25 mV, respectively, with PDI < 0.3. DOX-HA-Nio showed a good stability regarding size and charge over 4 weeks at 4 °C and maintain their integrity after lyophilization. HPLC results showed a 94.1±4.2 % encapsulation efficiency of DOX with good entrapment and slow, prolonged DOX release even after 48 hrs. Cell viability assay showed an IC of 14.26 nM for the DOX-HA-Nio against MCF-7 cell line with micromolar IC results against CD-44 negative cell lines (NIH/3T3). DOX-HA-Nio was proven to be an effective, targeted nanocarrier for DOX against MCF-7 cell line.
阿霉素（DOX）被广泛用于治疗实体瘤。 脂质体是由非离子表面活性剂自组装而成的纳米载体。 通过薄膜水化法制备了负载 DOX 的阳离子脂质体（DOX-Nio）。 然后，通过静电相互作用将透明质酸（DOX-HA-Nio）修饰到 DOX-Nio 上。 DOX-Nio 和 DOX-HA-Nio 的粒径分别为 120.0±1.02nm 和 182.9±2.3nm，表面电荷分别为+35.5±0.15mV 和-15.6±0.25mV，PDI<0.3。 DOX-HA-Nio 在 4°C 下 4 周内具有良好的稳定性，粒径和表面电荷变化不大，并在冻干后保持完整性。 HPLC 结果表明，DOX 的包封效率为 94.1±4.2%，具有良好的包封率和缓慢、持续的 DOX 释放，甚至在 48 小时后也是如此。 细胞活力测定表明，DOX-HA-Nio 对 MCF-7 细胞系的 IC 为 14.26nM，对 CD-44 阴性细胞系（NIH/3T3）的 IC 为毫摩尔级。 DOX-HA-Nio 被证明是一种有效的针对 MCF-7 细胞系的 DOX 靶向纳米载体。
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