College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China.
Department of Otolaryngology Head and Neck Surgery, Beijing TongRen Hospital, Capital Medical University, the Key Laboratory of Otolaryngology-Head and Neck Surgery (Ministry of Education of China), Beijing Key Laboratory of Nasal Diseases, Beijing Institute of Otolaryngology, Beijing, China.
Front Immunol. 2023 Dec 14;14:1290182. doi: 10.3389/fimmu.2023.1290182. eCollection 2023.
Cecropin AD (CAD), a renowned antimicrobial peptide, has shown promising potential in treating various bacterial infections. This study investigates the protective effects of CAD against lipopolysaccharide (LPS)-induced intestinal adversities in chickens.
Sixty SPF-grade chicks were divided into groups and exposed to different dosages of CAD, followed by LPS administration. The study assessed the impact of CAD on intestinal mucosal injury markers, oxidative stress, and inflammation.
LPS significantly increased Diamine oxidase (DAO) and D-lactate (D-LA) levels, both indicators of intestinal mucosal injury. CAD treatment substantially attenuated these elevations, particularly at higher dosages. Additionally, CAD markedly reduced oxidative stress in intestinal tissues, as shown by normalized antioxidant levels and decreased reactive oxygen species. Histological analysis supported these findings, showing better-preserved villi structures in CAD-treated groups. Furthermore, CAD significantly reduced IL-6 and IL-8 expression post-LPS stimulation and effectively regulated the NLRP3 inflammasome pathway, decreasing associated factors like NLRP3, Caspase-1, IL-1b, and IL-18.
The study demonstrates CAD's therapeutic potential in alleviating LPS-induced intestinal injuries. The protective effects are primarily attributed to its anti-inflammatory and antioxidative actions and modulation of the NLRP3 inflammasome pathway.
抗菌肽 Cecropin AD（CAD）在治疗各种细菌感染方面显示出了很大的潜力。本研究探讨了 CAD 对 LPS 诱导的鸡肠道损伤的保护作用。
将 60 只 SPF 级鸡分为几组，分别用不同剂量的 CAD 和 LPS 处理。本研究评估了 CAD 对肠道黏膜损伤标志物、氧化应激和炎症的影响。
LPS 显著增加了二胺氧化酶（DAO）和 D-乳酸（D-LA）的水平，这两个都是肠道黏膜损伤的指标。CAD 处理显著降低了这些升高，尤其是在高剂量时。此外，CAD 显著降低了肠道组织的氧化应激，表现为抗氧化水平的正常化和活性氧的减少。组织学分析支持了这些发现，表明 CAD 处理组的绒毛结构保存更好。此外，CAD 还显著降低了 LPS 刺激后的 IL-6 和 IL-8 的表达，并有效地调节了 NLRP3 炎性小体途径，降低了 NLRP3、Caspase-1、IL-1b 和 IL-18 等相关因素。
本研究表明 CAD 在减轻 LPS 诱导的肠道损伤方面具有治疗潜力。保护作用主要归因于其抗炎和抗氧化作用以及对 NLRP3 炎性小体途径的调节。
