Characterization of Proteins Extracted from sp., sp., and sp. Macroalgae Using Green Technology: Effect of Digestion on Antioxidant and ACE-I Inhibitory Activity.

Macroalgal proteins were extracted from (URPE) (green), (PPPE) (brown), and (LOPE) (red) using ultrasound-assisted enzymatic extraction, which is one of the green extraction technologies. Techno-functional, characteristic, and digestibility properties, and biological activities including antioxidant (AOA) and angiotensin-I converting enzyme (ACE-I) inhibitory activities were also investigated. According to the results, the extraction yield (EY) (94.74%) was detected in the extraction of , followed by and . PPPE showed the highest ACE-I inhibitory activity before digestion. In contrast to PPPE, LOPE (20.90 ± 0.00%) and URPE (20.20 ± 0.00%) showed higher ACE-I inhibitory activity after digestion. The highest total phenolic content (TPC) (77.86 ± 1.00 mg GAE/g) was determined in LOPE. On the other hand, the highest AOA (74.69 ± 1.78 mg TE/g) and AOA (251.29 ± 5.0 mg TE/g) were detected in PPPE. After digestion, LOPE had the highest TPC (22.11 ± 2.18 mg GAE/g), AOA (8.41 ± 0.06 mg TE/g), and AOA (88.32 ± 0.65 mg TE/g) ( < 0.05). protein digestibility of three macroalgal protein extracts ranged from 84.35 ± 2.01% to 94.09 ± 0.00% ( < 0.05). Three macroalgae showed high oil holding capacity (OHC), especially PPPE (410.13 ± 16.37%) ( < 0.05), but they showed minimum foaming and emulsifying properties. The quality of the extracted macroalgal proteins was assessed using FTIR, SDS-PAGE, and DSC analyses. According to our findings, the method applied for macroalgal protein extraction could have a potential the promise of ultrasonication application as an environmentally friendly technology for food industry. Moreover, URPE, PPPE, and LOPE from sustainable sources may be attractive in terms of nourishment for people because of their digestibility, antioxidant properties, and ACE-I inhibitory activities.