xTrans Corporate Research and Innovation Center, Taipei City, Taiwan.
Department of Chemistry, National Chung Hsing University, Taichung, Taiwan.
Int J Nanomedicine. 2023 Dec 28;18:8077-8097. doi: 10.2147/IJN.S440034. eCollection 2023.
Larger nanoparticles of bioactive compounds deposit high concentrations in follicular ducts after skin penetration. In this study, we investigated the effects of microcurrent cloth on the skin penetration and translocation of large nanoparticle applied for wound repair applications.
A self-assembly of curcumin-loaded micelles (CMs) was prepared to improve the water solubility and transdermal efficiency of curcumin. Microcurrent cloth (M) was produced by Zn/Ag electrofabric printing to facilitate iontophoretic transdermal delivery. The transdermal performance of CMs combined with M was evaluated by a transdermal system and confocal microscopy. The CMs/iontophoretic combination effects on nitric oxide (NO) production and inflammatory cytokines were evaluated in Raw 264.7 cells. The wound-healing property of the combined treatment was assessed in a surgically created full-thickness circular wound mouse model.
Energy-dispersive X-ray spectroscopy confirmed the presence of Zn/Ag on the microcurrent cloth. The average potential of M was measured to be +214.6 mV in PBS. Large particle CMs (CM-L) prepared using surfactant/cosurfactant present a particle size of 142.9 nm with a polydispersity index of 0.319. The solubility of curcumin in CM-L was 2143.67 μg/mL, indicating 250-fold higher than native curcumin (8.68 μg/mL). The combined treatment (CM-L+M) demonstrated a significant ability to inhibit NO production and increase IL-6 and IL-10 secretion. Surprisingly, microcurrent application significantly improved 20.01-fold transdermal performance of curcumin in CM-L with an obvious escape of CM-L from follicular ducts to surrounding observed by confocal microscopy. The CM-L+M group also exhibited a better wound-closure rate (77.94% on day 4) and the regenerated collagen intensity was approximately 2.66-fold higher than the control group, with a closure rate greater than 90% on day 8 in vivo.
Microcurrent cloth play as a promising iontophoretic transdermal drug delivery accelerator that enhances skin penetration and assists CMs to escape from follicular ducts for wound repair applications.
生物活性化合物的较大纳米颗粒在经皮穿透后会在滤泡导管中沉积高浓度。在这项研究中，我们研究了微电流布对用于伤口修复应用的大纳米颗粒应用的皮肤穿透和迁移的影响。
制备姜黄素载药胶束（CMs）自组装体以提高姜黄素的水溶性和经皮效率。通过 Zn/Ag 电纺印花生产微电流布（M）以促进离子电渗经皮给药。通过透皮系统和共聚焦显微镜评估 CMs 与 M 结合的经皮性能。在 Raw 264.7 细胞中评估 CMs/离子电渗联合对一氧化氮（NO）产生和炎症细胞因子的影响。在手术创建的全层圆形伤口小鼠模型中评估联合治疗的伤口愈合特性。
能谱仪证实微电流布上存在 Zn/Ag。在 PBS 中测量 M 的平均电位为+214.6 mV。使用表面活性剂/共表面活性剂制备的大颗粒 CMs（CM-L）的粒径为 142.9nm，多分散指数为 0.319。CM-L 中姜黄素的溶解度为 2143.67μg/mL，表明比天然姜黄素（8.68μg/mL）高 250 倍。联合治疗（CM-L+M）表现出显著抑制 NO 产生和增加 IL-6 和 IL-10 分泌的能力。令人惊讶的是，微电流应用显著提高了 CM-L 的经皮性能 20.01 倍，共聚焦显微镜观察到 CM-L 明显从滤泡导管逃逸到周围。CM-L+M 组的伤口闭合率也更高（第 4 天为 77.94%），再生胶原强度约为对照组的 2.66 倍，第 8 天体内闭合率大于 90%。
微电流布作为一种有前途的离子电渗经皮药物输送加速剂，可增强皮肤穿透并帮助 CMs 从滤泡导管逃逸，用于伤口修复应用。
