Jiangnan University Medical Center, Jiangnan University, Wuxi, China.
Central Laboratory, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Front Cell Infect Microbiol. 2023 Dec 18;13:1281745. doi: 10.3389/fcimb.2023.1281745. eCollection 2023.
( is a epidemiologically significant food-borne parasite, causing several hepatobiliary diseases. Biliary microbiota community structure might be influenced by infection with pathogens. However, the biliary microbiome of biliary obstruction patients infected with is still an unexplored aspect.
A total of 50 biliary obstruction patients were enrolled, including 24 infected with and 26 non-infected subjects. The bile samples were collected by Endoscopic Retrograde Cholangiopancretography. Biliary microbiota alteration was analyzed through high-throughput 16S ribosomal RNA (rRNA) gene sequencing.
Our findings revealed that there was significant increase in both richness and diversity, as well as changes in the taxonomic composition of the biliary microbiota of infected patients. At the phylum level, infection induced Proteobacteria increased and Firmicutes reduced. At the genus level, the relative abundance of and increased significantly, while decreased prominently in infected groups ( < 0.05). The PICRUSt analysis further showed remarkably different metabolic pathways between the two groups.
infection could modify the biliary microbiota, increasing the abundance and changing the phylogenetic composition of bacterial in biliary obstruction patients. This study may help deepen the understanding of the host-biliary microbiota interplay with infection on the background of biliary obstruction and provide new insights into understanding the pathogenesis of clonorchiasis.
（华支睾吸虫）是一种具有流行病学意义的食源性寄生虫，可引起多种肝胆疾病。胆道微生物群落结构可能受到病原体感染的影响。然而，感染 （华支睾吸虫）的胆道梗阻患者的胆道微生物组仍未得到充分研究。
共纳入 50 例胆道梗阻患者，包括 24 例感染 （华支睾吸虫）患者和 26 例非感染患者。通过内镜逆行胰胆管造影术采集胆汁样本。通过高通量 16S 核糖体 RNA（rRNA）基因测序分析胆道微生物群的变化。
我们的研究结果表明，感染患者的胆道微生物丰富度和多样性显著增加，且其分类组成发生变化。在门水平上，感染诱导的变形菌门增加，厚壁菌门减少。在属水平上， （华支睾吸虫）和 （副溶血弧菌）的相对丰度显著增加，而 （大肠埃希菌）的相对丰度明显降低（<0.05）。PICRUSt 分析进一步显示两组之间的代谢途径明显不同。
感染可能会改变胆道微生物群，增加细菌的丰度并改变胆道梗阻患者胆道中细菌的系统发育组成。本研究有助于加深对胆道梗阻背景下 （华支睾吸虫）感染宿主-胆道微生物相互作用的理解，并为理解华支睾吸虫病的发病机制提供新的见解。
