草鱼呼肠孤病毒VP56和VP35诱导形成用于复制的病毒包涵体。
Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication.
作者信息
Zhang Chu, Wu Hui, Feng Hao, Zhang Yong-An, Tu Jiagang
机构信息
State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan, China.
State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, China.
出版信息
iScience. 2023 Dec 7;27(1):108684. doi: 10.1016/j.isci.2023.108684. eCollection 2024 Jan 19.
Viral inclusion bodies (VIBs) are subcellular structures required for efficient viral replication. How type II grass carp reovirus (GCRV-II), the mainly prevalent strain, forms VIBs is unknown. In this study, we found that GCRV-II infection induced punctate VIBs in grass carp ovary (GCO) cells and that non-structural protein 38 (NS38) functioned as a participant in VIB formation. Furthermore, VP56 and VP35 induced VIBs and recruited other viral proteins via the N-terminal of VP56 and the middle domain of VP35. Additionally, we found that the newly synthesized viral RNAs co-localized with VP56 and VP35 in VIBs during infection. Taken together, VP56 and VP35 induce VIB formation and recruit other viral proteins and viral RNAs to the VIBs for viral replication, which helps identify new targets for developing anti-GCRV-II drugs to disrupt viral replication.
病毒包涵体（VIBs）是高效病毒复制所需的亚细胞结构。主要流行毒株II型草鱼呼肠孤病毒（GCRV-II）如何形成VIBs尚不清楚。在本研究中，我们发现GCRV-II感染在草鱼卵巢（GCO）细胞中诱导点状VIBs形成，并且非结构蛋白38（NS38）作为VIB形成的参与者发挥作用。此外，VP56和VP35诱导VIBs形成，并通过VP56的N端和VP35的中间结构域招募其他病毒蛋白。此外，我们发现在感染期间新合成的病毒RNA与VP56和VP35在VIBs中共定位。综上所述，VP56和VP35诱导VIBs形成，并将其他病毒蛋白和病毒RNA招募到VIBs中进行病毒复制，这有助于确定开发抗GCRV-II药物以破坏病毒复制的新靶点。
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