Talearngkul Rinrada, Sae-Tan Sudathip, Sirivarasai Jintana
Master of Science Program in Nutrition, Faculty of Medicine Ramathibodi Hospital and Institute of Nutrition, Mahidol University, Bangkok 10400, Thailand.
Department of Food Science and Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
Foods. 2023 Dec 4;12(23):4373. doi: 10.3390/foods12234373.
Probiotics can ameliorate type 2 diabetes mellitus (T2DM) via several mechanisms such as by decreasing inflammatory cytokines and increasing pancreatic β-cell functions. Another targeted mechanism for managing T2DM involves inhibiting α-amylase and α-glucosidase, which exhibit antioxidant activity and affect carbohydrate metabolism by delaying carbohydrate digestion, thus mitigating glucose in the circulation. Dairy products are effective matrices for delivering probiotics through the gastrointestinal tract. We compared the viability and antioxidant activity of the probiotics , , and subsp. in yogurt ice cream after in vitro digestion and compared α-amylase and α-glucosidase inhibition activities. had the highest viability after in vitro digestion (oral, gastric, and intestinal). and exhibited the highest percentages of α-glucosidase (16.37% ± 0.32%) and α-amylase (41.37% ± 0.61%) inhibition. subsp. and showed the highest antioxidant activities via the α,α-diphenyl-β-picrylhydrazyl free radical-scavenging method and ferric-reducing antioxidant power assay, respectively. These findings suggest that yogurt ice cream can provide a suitable matrix for the delivery of probiotics from dairy culture to promote intestinal homeostasis with probiotic benefits in the host as well as a potential functional food to help reduce postprandial hyperglycaemia.
益生菌可通过多种机制改善2型糖尿病(T2DM),例如降低炎性细胞因子水平和增强胰腺β细胞功能。另一种治疗T2DM的靶向机制涉及抑制α-淀粉酶和α-葡萄糖苷酶,它们具有抗氧化活性,并通过延缓碳水化合物消化来影响碳水化合物代谢,从而减轻循环中的葡萄糖水平。乳制品是将益生菌输送至胃肠道的有效载体。我们比较了益生菌、和亚种在体外消化后的酸奶冰淇淋中的活力和抗氧化活性,并比较了它们对α-淀粉酶和α-葡萄糖苷酶的抑制活性。在体外消化(口腔、胃和肠道)后活力最高。和对α-葡萄糖苷酶(16.37%±0.32%)和α-淀粉酶(41.37%±0.61%)的抑制率最高。亚种和分别通过α,α-二苯基-β-苦味酰肼自由基清除法和铁还原抗氧化能力测定法显示出最高的抗氧化活性。这些研究结果表明,酸奶冰淇淋可以作为一种合适的载体,将乳制品培养的益生菌输送至肠道,促进宿主肠道内的益生菌平衡,同时也是一种潜在的功能性食品,有助于降低餐后高血糖。