Devi Kangjam Rekha, Deka Archana, Mukherjee Debdutta, Kaur Harpreet, Narain Kanwar
Indian Council of Medical Research-Regional Medical Research Centre, Dibrugarh 786001, Assam, India.
Indian Council of Medical Research-Headquarters, New Delhi 110029, India.
Trop Med Infect Dis. 2023 Dec 22;9(1):6. doi: 10.3390/tropicalmed9010006.
Human pulmonary paragonimiasis, an emerging concern in North East India, frequently masquerades as pulmonary tuberculosis due to clinical and radiological similarities, leading to diagnostic challenges. This research aimed to harness the immunoblotting technique to discern immunodiagnostic protein antigens from both adult worm and excretory-secretory (ES) extracts of the prevalent type 1 in Arunachal Pradesh, North East India. We studied the time kinetics of immunoreactive patterns in relation to the duration of infection in rodent models. Immunoblot analyses were also conducted using sera from ELISA-positive patients confirmed with paragonimiasis, facilitating the selection of antigenic extracts with diagnostic potential. Further, ES protein antigens were subjected to 2D immunoblot analysis and immunoreactive protein spots identified using MALDI-TOF MS. The immunoreactivity patterns of ES antigens with sera of paragonimiasis-positive patients were detailed, and specific immunoreactive protein antigens were pinpointed using peptide mass fingerprinting (MALDI-TOF). This work underscores the enhanced diagnostic accuracy when combining ELISA with immunoblotting for pulmonary paragonimiasis in regions like North East India, marked by co-existing helminth infections.
人类肺吸虫病在印度东北部日益受到关注，由于临床和影像学表现相似，常被误诊为肺结核，给诊断带来挑战。本研究旨在利用免疫印迹技术，从印度东北部阿鲁纳恰尔邦流行的1型成虫和排泄分泌（ES）提取物中识别免疫诊断蛋白抗原。我们研究了啮齿动物模型中免疫反应模式与感染持续时间的时间动力学关系。还使用经肺吸虫病确诊的ELISA阳性患者的血清进行免疫印迹分析，以筛选具有诊断潜力的抗原提取物。此外，对ES蛋白抗原进行二维免疫印迹分析，并用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定免疫反应性蛋白斑点。详细阐述了ES抗原与肺吸虫病阳性患者血清的免疫反应模式，并使用肽质量指纹图谱（MALDI-TOF）确定了特异性免疫反应性蛋白抗原。这项工作强调，在印度东北部等存在多种蠕虫感染的地区，将ELISA与免疫印迹相结合用于肺吸虫病诊断时，可提高诊断准确性。
