从印度一个有组织的山羊群中分离出的S型的全基因组序列。
Complete genome sequence of S-type isolated from an organized goat herd in India.
作者信息
Singh Vinod Kumar, Das Chayanika, Kumar Amit, Prajapati Malyaj R, Yadav Sharad Kumar
机构信息
Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, U.P. Pt. Deen Dayal Uphadhayay Pashu Chikitsa Vigyan Viswavidhyalay Evam Go-Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh India.
Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh India.
出版信息
3 Biotech. 2024 Feb;14(2):38. doi: 10.1007/s13205-023-03896-2. Epub 2024 Jan 17.
The present report communicates the first complete genome sequence of S-type , isolated from an organised goat herd in Uttar Pradesh, India. Bacteria were isolated in pure culture on Herrold's egg yolk medium (HEYM) slants containing mycobactin J from the faecal sample collected per-rectally from a clinical diseased goat, and next-generation sequencing (NGS) revealed that the genome sequence length of the isolated strain named MAP-Gt-9 is 4,509,428 bp with no plasmid DNA, with a GC content of 69.5%, an N50 value of 125,474 bp, and an L50 value of 12, containing 4235 coding DNA sequences (CDSs), 44 tRNAs, 3 ncRNAs and 1 each 5S, 16S, 23S rRNA genes.
本报告公布了从印度北方邦一个有组织的山羊群中分离出的S型的首个完整基因组序列。从一头临床患病山羊经直肠采集的粪便样本中，在含有分枝杆菌素J的赫罗尔德蛋黄培养基（HEYM）斜面上进行纯培养分离出细菌，下一代测序（NGS）显示，分离菌株MAP-Gt-9的基因组序列长度为4,509,428 bp，无质粒DNA，GC含量为69.5%，N50值为125,474 bp，L50值为12，包含4235个编码DNA序列（CDS）、44个tRNA、3个非编码RNA以及各1个5S、16S、23S rRNA基因。
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