PC-H1 来源的细胞外囊泡抑制结肠癌中 HIF-1α 介导的糖酵解。
Extracellular vesicles from PC-H1 inhibit HIF-1α-mediated glycolysis of colon cancer.
机构信息
Department of Microbiology, Harbin Medical University, 150081 Harbin, China.
Department of Microbiology, Beihua University, 132013 Jilin, China.
出版信息
Future Microbiol. 2024 Feb;19:227-239. doi: 10.2217/fmb-2023-0144. Epub 2024 Jan 25.
Extracellular vesicles from PC-H1 have antiproliferative activity of colon cells, but the effect on glycolytic metabolism of cancer cell remains enigmatic. The authors investigated how extracellular vesicles (LpEVs) inhibit the growth of colon cancer cells by affecting tumor metabolism. HCT116 cells were treated with LpEVs and then differentially expressed genes were analyzed by transcriptome sequencing, the sequencing results were confirmed and . LpEVs entered colon cancer cells and inhibited their growth. Transcriptome sequencing revealed differentially expressed genes were related to glycolysis. Lactate production, glucose uptake and lactate dehydrogenase activity were significantly reduced after treatment. LpEVs also reduced HIF-1α, GLUT1 and LDHA expression. LpEVs exert their antiproliferative activity of colon cancer cells by decreasing HIF-1α-mediated glycolysis.
PC-H1 来源的细胞外囊泡具有抗结肠细胞增殖活性，但对癌细胞糖酵解代谢的影响仍不清楚。作者研究了细胞外囊泡 (LpEVs) 通过影响肿瘤代谢来抑制结肠癌细胞生长的方式。用 LpEVs 处理 HCT116 细胞，然后通过转录组测序分析差异表达基因，测序结果得到验证。LpEVs 进入结肠癌细胞并抑制其生长。转录组测序显示差异表达基因与糖酵解有关。处理后乳酸生成、葡萄糖摄取和乳酸脱氢酶活性显著降低。LpEVs 还降低了 HIF-1α、GLUT1 和 LDHA 的表达。LpEVs 通过降低 HIF-1α 介导的糖酵解发挥其抗结肠癌细胞增殖活性。
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