RNF213 抑制肺腺癌细胞的迁移。

RNF213 inhibits migration in lung adenocarcinoma cell.

机构信息

Department of Oncology, The fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, China.

Department of Pathology, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai 519000, China.

出版信息

Cell Mol Biol (Noisy-le-grand). 2023 Dec 20;69(14):286-292. doi: 10.14715/cmb/2023.69.14.47.

DOI:10.14715/cmb/2023.69.14.47

PMID:38279417

Abstract

Non-small cell lung cancer (NSCLC) is one of the most common malignant tumors, and lung adenocarcinoma (LUAD) accounts for up to 40% of NSCLC. Ring finger protein 213(RNF213) has been demonstrated to suppress several cancers, including glioblastoma and breast cancer. Nonetheless, the role of RNF213 in LUAD has not been investigated. The expression of RNF213 in LUAD tissues was analyzed by western blotting, The Cancer Genome Atlas, Genotype Tissue Expression Project, and Gene Expression Omnibus databases. Prognostic value analysis was performed through the Kaplan-Meier Plotter database. We determined the role of RNF213 in LUAD cells through cell counting kit‑8 assay, migration, and invasion assay. The clinical roles of RNF213 were evaluated by immunohistochemical staining assay (IHC) and Kaplan-Meier survival analysis. RNF213 expression was reduced in LUAD, thus affecting the prognosis of LUAD. And RNF213 could suppress the migration and invasion of LUAD cells to prevent tumor development. the expression of RNF213 is positively correlated with the overall survival, providing a novel marker in the prognosis of LUAD patients.

摘要

非小细胞肺癌（NSCLC）是最常见的恶性肿瘤之一，肺腺癌（LUAD）占 NSCLC 的 40%以上。环指蛋白 213（RNF213）已被证明可抑制多种癌症，包括神经胶质瘤和乳腺癌。然而，RNF213 在 LUAD 中的作用尚未得到研究。通过 Western blot、The Cancer Genome Atlas、Genotype Tissue Expression Project 和 Gene Expression Omnibus 数据库分析 LUAD 组织中 RNF213 的表达。通过 Kaplan-Meier Plotter 数据库进行预后价值分析。我们通过细胞计数试剂盒-8 测定法、迁移和侵袭测定法确定 RNF213 在 LUAD 细胞中的作用。通过免疫组织化学染色测定法（IHC）和 Kaplan-Meier 生存分析评估 RNF213 的临床作用。RNF213 在 LUAD 中的表达降低，从而影响 LUAD 的预后。并且 RNF213 可以抑制 LUAD 细胞的迁移和侵袭，从而防止肿瘤发展。RNF213 的表达与总生存期呈正相关，为 LUAD 患者的预后提供了一个新的标志物。