Department of Clinical Pharmacy, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Department of Respiratory and geriatrics, Honghui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Int Immunopharmacol. 2024 Feb 15;128:111583. doi: 10.1016/j.intimp.2024.111583. Epub 2024 Jan 28.
Crocetin is a kind of glycocone naturally occurring in Crocus sativus L.. It is an active metabolite produced by biohydrolysis of Crocus sativus L.. Crocetin has anti-cardiovascular diseases and antioxidant effects, but its anti-allergic effect has not been reported. In this study, the inhibitory effect of crocetin on immunoglobulin E (IgE) - mediated allergic reaction and the mechanism of action were investigated. The passive cutaneous anaphylaxis (PCA) was used to elucidate the anti-allergic effects of crocetin in vivo. Degranulation assay, calcium imaging, and cytokine release assay were to evaluate the anti-allergic effect of crocetin in vitro. We found that crocetin IgE-mediated RBL-2H3 cell degranulation and allergy both in vitro and in vivo. The TNF pathway was inhibited by crocetin in our RNA-seq sequences, Furthermore, crocetin inhibits IgE-mediated calcium influx, and PLC / IP3 phosphorylation in RBL-2H3 cells. Our findings suggested that crocetin revealed prominent anti-allergy activity through TNF and Ca/PLC/IP3 pathway.
西红花酸是一种天然存在于藏红花中的甘波聚糖。它是藏红花生物水解产生的一种活性代谢物。西红花酸具有抗心血管疾病和抗氧化作用，但尚未有其抗过敏作用的报道。在本研究中，研究了西红花酸对免疫球蛋白 E（IgE）介导的过敏反应的抑制作用及其作用机制。通过被动皮肤过敏反应（PCA）阐明了西红花酸在体内的抗过敏作用。脱颗粒测定、钙成像和细胞因子释放测定用于评估西红花酸的体外抗过敏作用。我们发现西红花酸在体内和体外均可抑制 IgE 介导的 RBL-2H3 细胞脱颗粒和过敏反应。我们的 RNA-seq 序列表明，西红花酸抑制 TNF 通路。此外，西红花酸抑制 IgE 介导的 RBL-2H3 细胞内钙离子内流和 PLC/IP3 磷酸化。我们的研究结果表明，西红花酸通过 TNF 和 Ca/PLC/IP3 途径显示出显著的抗过敏活性。
