MST1 和 MST2 基因单核苷酸多态性与幽门螺杆菌感染及非贲门胃腺癌发生的相关性研究

Association Study between SNPs in MST1 and MST2 and H. pylori Infection as well as Noncardia Gastric Carcinogenesis.

机构信息

School of Basic Medicine and Forensic Medicine, Baotou Medical College, Baotou, China.

School of Medical Technology and Anesthesiology, Baotou Medical College, Baotou, China.

出版信息

Dig Dis. 2024;42(3):230-239. doi: 10.1159/000536507. Epub 2024 Jan 31.

DOI:10.1159/000536507

PMID:38295774

Abstract

INTRODUCTION

Gastric cancer (GC) remains a global health challenge, and H. pylori infection is a main risk factor for noncardia GC. The present study aimed to investigate the association between single nucleotide polymorphisms (SNPs) in mammalian sterile 20-like kinase 1 (MST1) and MST2, H. pylori (H. pylori) infection, and the risk of noncardia gastric cancer (GC).

METHODS

A case-control study was conducted using enzyme-linked immunosorbent assay (ELISA) and TaqMan method to detect the titer of anti-H. pylori antibody in normal human serum and genotype 9 SNPs of MST1 and MST2 genes among 808 samples. Unconditional logistic regression was used to calculate odds ratios (ORs) and 95% confidence intervals (CIs) for the association between SNPs and H. pylori infection, as well as the risk of noncardia gastric cancer in codominant, dominant, overdominant, recessive, and log-additive genetic models. Haplotypes were constructed using the Haploview 4.2 software.

RESULTS

The CC genotype of MST2 SNP rs10955176 was associated with a reduced risk of H. pylori infection compared to the TT + CT genotype. None of other SNPs were associated with H. pylori infection. The TT genotype of MST2 SNP rs7827435 was associated with a reduced risk of noncardia gastric cancer compared to the AA + AT genotype. None of the SNPs were associated with noncardia gastric cancer. There were no associations between haplotypes and H. pylori infection or the risk of noncardia gastric cancer.

CONCLUSIONS

The CC genotype of rs10955176 and the TT genotype of rs7827435 may serve as protective factors against H. pylori infection and noncardia gastric cancer risk, respectively.

摘要

简介

胃癌（GC）仍然是一个全球性的健康挑战，幽门螺杆菌（H. pylori）感染是导致非贲门 GC 的主要危险因素。本研究旨在探讨哺乳动物无 sterile 20 样激酶 1（MST1）和 MST2 单核苷酸多态性（SNPs）与 H. pylori 感染以及非贲门胃癌（GC）风险之间的关系。

方法

采用酶联免疫吸附试验（ELISA）和 TaqMan 法检测 808 例正常人血清抗 H. pylori 抗体滴度和 MST1、MST2 基因 9 号 SNP 基因型，采用非条件 logistic 回归计算 SNP 与 H. pylori 感染及非贲门 GC 风险的关联的优势比（ORs）及其 95%置信区间（CIs），并采用共显性、显性、超显性、隐性和对数相加遗传模型进行分析。采用 Haploview 4.2 软件构建单体型。

结果

与 TT + CT 基因型相比，MST2 SNP rs10955176 的 CC 基因型与 H. pylori 感染风险降低相关。其他 SNP 与 H. pylori 感染无关。与 AA + AT 基因型相比，MST2 SNP rs7827435 的 TT 基因型与非贲门 GC 风险降低相关。其他 SNP 与非贲门 GC 无关。单体型与 H. pylori 感染或非贲门 GC 风险无关。