Department of Burns, The First Affiliated Hospital of Anhui Medical University, Hefei, 230032, China.
School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Medical University, Hefei, 230032, China.
Adv Healthc Mater. 2024 Jun;13(16):e2303599. doi: 10.1002/adhm.202303599. Epub 2024 Feb 20.
Free radicals are secreted following skin damage and cause oxidative stress and inflammatory reactions that increase the difficulty of wound healing. In this study, copper-based nanozyme CuSe nanosheets (NSs) are synthesized by an anion-exchange strategy and apply to wounds with F127 hydrogels to investigate the healing effect of this nanozyme composite hydrogels on wounds. CuSe NSs have a large number of catalytically active centers, are simple to synthesize, require few reaction conditions and have a short synthesis cycle. In vitro experiments have shown that CuSe NSs possess superoxide dismutase (SOD)-like activity and nitrogen radical scavenging activity and promote angiogenesis and fibroblast migration. The doping of CuSe NSs into the F127 hydrogel does not have a significantly affect on the properties of the hydrogel. This hybridized hydrogel not only adapts to the irregular and complex morphology of acute wounds but also prolongs the duration of nanozyme action on the wound, thus promoting wound healing. Transcriptomic analysis further reveals the potential therapeutic mechanism of the CuSe/F127 hydrogel in promoting acute wound healing. Animal experiments have shown that the CuSe/F127 hydrogel has good biosafety. The CuSe/F127 hydrogel provides an innovative idea for the development of hydrogel dressings for the treatment of acute wounds.
自由基会在皮肤损伤后分泌出来，导致氧化应激和炎症反应，增加伤口愈合的难度。在这项研究中，采用阴离子交换策略合成了基于铜的纳米酶 CuSe 纳米片（NSs），并将其应用于 F127 水凝胶伤口，以研究这种纳米酶复合水凝胶对伤口的愈合效果。CuSe NSs 具有大量的催化活性中心，合成简单，反应条件要求少，合成周期短。体外实验表明，CuSe NSs 具有超氧化物歧化酶（SOD）样活性和氮自由基清除活性，促进血管生成和成纤维细胞迁移。CuSe NSs 掺杂到 F127 水凝胶中不会显著影响水凝胶的性能。这种杂交水凝胶不仅适应急性伤口不规则和复杂的形态，还延长了纳米酶在伤口上的作用时间，从而促进伤口愈合。转录组分析进一步揭示了 CuSe/F127 水凝胶在促进急性伤口愈合方面的潜在治疗机制。动物实验表明，CuSe/F127 水凝胶具有良好的生物安全性。CuSe/F127 水凝胶为治疗急性伤口的水凝胶敷料的开发提供了新的思路。
