长链非编码 RNA WWTR1-AS1 通过 miR-136 上调 Notch3 以增加宫颈鳞状细胞癌中的癌细胞干性。
LncRNA WWTR1-AS1 upregulates Notch3 through miR-136 to increase cancer cell stemness in cervical squamous cell carcinoma.
机构信息
Department of Obstetrics and Gynecology, Bishan hospital of Chongqing medical university, Bishan Hospital of Chongqing, No. 9 Shuangxing Avenue, Biquan Street, Bishan District, 402760, Chongqing City, P. R. China.
出版信息
BMC Womens Health. 2024 Feb 8;24(1):104. doi: 10.1186/s12905-024-02905-7.
BACKGROUND
This Study investigated the role of WWTR1-AS1 in cervical squamous cell carcinoma (CSCC).
RESULTS
WWTR1-AS1 expression was upregulated in CSCC tissues. WWTR1-AS1 was predicted to interact with miR-136, whereas correlation analysis revealed that there was no close correlation between WWTR1-AS1 and miR-136 across CSCC samples. Moreover, WWTR1-AS1 and miR-136 did not regulate the expression of each other. In addition, overexpression of WWTR1-AS1 increased the expression levels of Notch3, which could be targeted by miR-136. Cell stemness analysis indicated that the overexpression of WWTR1-AS1 and Notch3 increased CSCC cell stemness and the capacity of CSCC cell to grow as spheroids. Overexpression of miR-136 decreased CSCC cell stemness and reversed the effects of overexpression of WWTR1-AS1 on Notch3 in CSCC cells.
CONCLUSION
Therefore, WWTR1-AS1 may upregulate Notch3 through miR-136 to increase cancer cell stemness in CSCC.
背景
本研究探讨了 WWTR1-AS1 在宫颈鳞状细胞癌（CSCC）中的作用。
结果
WWTR1-AS1 在 CSCC 组织中表达上调。WWTR1-AS1 被预测与 miR-136 相互作用，而相关性分析表明，CSCC 样本中 WWTR1-AS1 与 miR-136 之间没有密切的相关性。此外，WWTR1-AS1 和 miR-136 并没有相互调节对方的表达。此外，WWTR1-AS1 的过表达增加了 Notch3 的表达水平，而 Notch3 可以被 miR-136 靶向。细胞干性分析表明，WWTR1-AS1 和 Notch3 的过表达增加了 CSCC 细胞的干性和形成球体的能力。miR-136 的过表达降低了 CSCC 细胞的干性，并逆转了 WWTR1-AS1 过表达对 CSCC 细胞中 Notch3 的影响。
结论
因此，WWTR1-AS1 可能通过 miR-136 上调 Notch3，从而增加 CSCC 中癌细胞的干性。
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