利用褐腐真菌和丝状真菌混合培养物对亚甲基蓝进行生物脱色和生物转化：代谢产物鉴定及降解途径
Biodecolorization and biotransformation of methylene blue using mixed cultures of brown-rot fungus and filamentous fungus : identification of metabolites and degradation pathway.
作者信息
Purnomo Adi Setyo, Fauzany Umirul Solichah, Rizqi Hamdan Dwi, Alkas Taufiq Rinda, Kamei Ichiro
机构信息
Department of Chemistry, Institut Teknologi Sepuluh Nopember (ITS) Kampus ITS Sukolilo Surabaya 60111 Indonesia
Department of Environment Management, Politeknik Pertanian Negeri Samarinda Samarinda 75131 Indonesia.
出版信息
RSC Adv. 2024 Feb 8;14(8):5061-5068. doi: 10.1039/d3ra08544a. eCollection 2024 Feb 7.
This study aimed to examine biodecolorization and biotransformation of methylene blue (MB) using mixed cultures of brown-rot fungus and filamentous fungus . In addition, the ratio of and in mixed cultures was 1 : 1, and the sample was incubated at 30 °C for 7 days in liquid medium potato dextrose broth (PDB). The results showed that the sample had the ability to remove and transform 95.24 mg L MB. In this study, mixed cultures had the highest removal percentage of 64.77%, while values of 5.94% and 36.82% were obtained for single cultures of and , respectively. LC-TOF/MS analysis results showed that peak intensity of MB compound (/ 284) in each treatment chromatogram decreased compared to the control. The metabolites of decolorization by were CHNS, CHNSO, and CHNSO, while CHNS was obtained using . For mixed cultures, the metabolites obtained included CHNOS, CHNOS, CHNOS, and CHNS. Based on the results, mixed cultures of and had a high MB decolorization and could be used in the textile industry.
本研究旨在考察褐腐真菌和丝状真菌混合培养物对亚甲基蓝（MB）的生物脱色和生物转化作用。此外，混合培养物中二者比例为1∶1，样品在液体培养基马铃薯葡萄糖肉汤（PDB）中于30℃培养7天。结果表明，该样品具有去除和转化95.24 mg/L MB的能力。本研究中，混合培养物的去除率最高，为64.77%，而褐腐真菌和丝状真菌单一培养物的去除率分别为5.94%和36.82%。液相色谱-飞行时间质谱（LC-TOF/MS）分析结果表明，与对照相比，各处理色谱图中亚甲基蓝化合物（m/z 284）的峰强度降低。褐腐真菌脱色的代谢产物为CHNS、CHNSO和CHNSO，而丝状真菌脱色得到的代谢产物为CHNS。对于混合培养物，得到的代谢产物包括CHNOS、CHNOS、CHNOS和CHNS。基于这些结果，褐腐真菌和丝状真菌的混合培养物具有较高的亚甲基蓝脱色能力，可用于纺织工业。
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