用于翻译后修饰的定点安装的正交翻译。
Orthogonal Translation for Site-Specific Installation of Post-translational Modifications.
机构信息
Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, Arkansas 72701, United States.
Cell and Molecular Biology Program, University of Arkansas, Fayetteville, Arkansas 72701, United States.
出版信息
Chem Rev. 2024 Mar 13;124(5):2805-2838. doi: 10.1021/acs.chemrev.3c00850. Epub 2024 Feb 19.
Post-translational modifications (PTMs) endow proteins with new properties to respond to environmental changes or growth needs. With the development of advanced proteomics techniques, hundreds of distinct types of PTMs have been observed in a wide range of proteins from bacteria, archaea, and eukarya. To identify the roles of these PTMs, scientists have applied various approaches. However, high dynamics, low stoichiometry, and crosstalk between PTMs make it almost impossible to obtain homogeneously modified proteins for characterization of the site-specific effect of individual PTM on target proteins. To solve this problem, the genetic code expansion (GCE) strategy has been introduced into the field of PTM studies. Instead of modifying proteins after translation, GCE incorporates modified amino acids into proteins during translation, thus generating site-specifically modified proteins at target positions. In this review, we summarize the development of GCE systems for orthogonal translation for site-specific installation of PTMs.
翻译后修饰 (PTMs) 赋予蛋白质新的特性，以响应环境变化或生长需求。随着先进的蛋白质组学技术的发展，在细菌、古菌和真核生物的各种蛋白质中已经观察到数百种不同类型的 PTMs。为了确定这些 PTMs 的作用，科学家们已经应用了各种方法。然而，PTMs 的高动态性、低化学计量和相互作用使得几乎不可能获得均一修饰的蛋白质，从而无法对单个 PTM 对靶蛋白的特定位置的影响进行特征描述。为了解决这个问题，遗传密码扩展 (GCE) 策略已经被引入 PTM 研究领域。GCE 不是在翻译后修饰蛋白质，而是在翻译过程中将修饰的氨基酸掺入蛋白质中，从而在靶位置生成特异性修饰的蛋白质。在这篇综述中，我们总结了用于正交翻译的 GCE 系统的发展，用于在特定位置安装 PTMs。
Zotero 插件