Tomczyk Maja, Bidzan-Wiącek Monika, Kortas Jakub Antoni, Kochanowicz Magdalena, Jost Zbigniew, Fisk Helena L, Calder Philip C, Antosiewicz Jędrzej
Department of Biochemistry, Gdansk University of Physical Education and Sport, 80-336, Gdansk, Poland.
Department of Bioenergetics and Physiology of Exercise, Medical University of Gdansk, 80-211, Gdansk, Poland.
Sci Rep. 2024 Feb 19;14(1):4102. doi: 10.1038/s41598-024-54112-x.
The effects of long-term omega-3 polyunsaturated fatty acid (n-3 PUFA) supplementation during endurance training on tryptophan (Trp) metabolism and mental state of healthy individuals have not been evaluated so far. Concentrations of plasma Trp, its metabolites and IL-6 were assessed in 26 male runners before and after a 12-week training program combined with supplementation of n-3 PUFAs (O-3 + TRAIN group) or medium chain triglycerides (MCTs; TRAIN group). After the 12-week program participants' mood before and after stress induction was also assessed. The effects of the same supplementation protocol were evaluated also in 14 inactive subjects (O-3 + SEDEN group). Concentrations of 3-hydroxykynurenine (3-HK) and picolinic acid (PA) significantly increased only in the O-3 + TRAIN group (p = 0.01; [Formula: see text] = 0.22 and p = 0.01; [Formula: see text]= 0.26). Favorable, but not statistically significant changes in the concentrations of kynurenic acid (KYNA) (p = 0.06; [Formula: see text]= 0.14), xanthurenic acid (XA) (p = 0.07; [Formula: see text]= 0.13) and 3-hydroxyanthranilic acid (3-HAA) (p = 0.06; [Formula: see text]= 0.15) and in the ratio of neurotoxic to neuroprotective metabolites were seen also only in the O-3 + TRAIN group. No changes in mood and IL-6 concentrations were observed in either group. Supplementation with n-3 PUFAs during endurance training has beneficial effects on Trp's neuroprotective metabolites.Trial registry: This study was registered at ClinicalTrials.gov with identifier NCT05520437 (14/07/2021 first trial registration and 2018/31/N/NZ7/02962 second trial registration).
迄今为止，尚未评估在耐力训练期间长期补充ω-3多不饱和脂肪酸（n-3 PUFA）对健康个体色氨酸（Trp）代谢和精神状态的影响。在26名男性跑步者中，评估了一项为期12周的训练计划（结合n-3多不饱和脂肪酸补充剂（O-3+TRAIN组）或中链甘油三酯（MCTs；TRAIN组））前后血浆Trp、其代谢产物和IL-6的浓度。在12周计划结束后，还评估了参与者在应激诱导前后的情绪。在14名不运动的受试者（O-3+SEDEN组）中也评估了相同补充方案的效果。仅在O-3+TRAIN组中，3-羟基犬尿氨酸（3-HK）和吡啶甲酸（PA）的浓度显著增加（p=0.01；[公式：见原文]=0.22和p=0.01；[公式：见原文]=0.26）。犬尿喹啉酸（KYNA）（p=0.06；[公式：见原文]=0.14）、黄尿酸（XA）（p=0.07；[公式：见原文]=0.13）和3-羟基邻氨基苯甲酸（3-HAA）（p=0.06；[公式：见原文]=0.15）的浓度以及神经毒性与神经保护性代谢产物的比例也仅在O-3+TRAIN组中出现了有利但无统计学意义的变化。两组的情绪和IL-6浓度均未观察到变化。在耐力训练期间补充n-3多不饱和脂肪酸对Trp的神经保护性代谢产物有有益影响。试验注册：本研究在ClinicalTrials.gov上注册，标识符为NCT05520437（首次试验注册于2021年7月14日，第二次试验注册于2018/31/N/NZ7/02962）。
