幽门螺杆菌上调 circPGD，促进胃癌的发展。
Helicobacter pylori upregulates circPGD and promotes development of gastric cancer.
机构信息
The Affiliated Yixing Hospital of Jiangsu University, Wuxi, 214200, Jiangsu, China.
Urology Department, The Affiliated Taizhou Second People's Hospital of Yangzhou University, Taizhou, 225500, Jiangsu, China.
出版信息
J Cancer Res Clin Oncol. 2024 Feb 26;150(2):104. doi: 10.1007/s00432-023-05537-w.
PURPOSE
Helicobacter pylori (H. pylori) has unique biochemical traits and pathogenic mechanisms, which make it a substantial cause of gastrointestinal cancers. Circular RNAs (circRNAs) have concurrently been identified as an important participating factor in the pathophysiology of several different cancers. However, the underlying processes and putative interactions between H. pylori and circRNAs have received very little attention. To address this issue, we explored the interaction between H. pylori and circRNAs to investigate how they might jointly contribute to the occurrence and development of gastric cancer.
METHODS
Changes in circPGD expression in H. pylori were detected using qRT-PCR. Cell proliferation and migration changes were assayed by colony formation, the CCK-8 assay and the transwell assay. Apoptosis was measured by flow cytometry. Western blot was conducted to detect changes in cell migration, apoptosis, proliferation and inflammation-associated proteins. QRT-PCR was used to measure changes in circPGD and inflammation-associated factors.
RESULTS
We found that H. pylori induced increased circPGD expression in infected human cells and facilitated gastric cancer progression in three ways by promoting cell proliferation and migration, enhancing the inflammatory response, and inhibiting apoptosis.
CONCLUSIONS
CircPGD appears to play a role in H. pylori-related gastric cancer and may thus be a viable, novel target for therapeutic intervention.
目的
幽门螺杆菌（H. pylori）具有独特的生化特性和发病机制，是胃肠道癌症的重要病因。环状 RNA（circRNA）也被认为是多种癌症病理生理学的一个重要参与因素。然而，H. pylori 与 circRNA 之间的潜在过程和假定相互作用尚未得到充分关注。为了解决这一问题，我们探讨了 H. pylori 与 circRNAs 之间的相互作用，以研究它们如何共同促进胃癌的发生和发展。
方法
通过 qRT-PCR 检测 H. pylori 中 circPGD 的表达变化。通过集落形成、CCK-8 检测和 Transwell 检测来检测细胞增殖和迁移变化。通过流式细胞术测量细胞凋亡。通过 Western blot 检测细胞迁移、凋亡、增殖和炎症相关蛋白的变化。通过 qRT-PCR 检测 circPGD 和炎症相关因子的变化。
结果
我们发现 H. pylori 诱导感染细胞中 circPGD 的表达增加，并通过促进细胞增殖和迁移、增强炎症反应和抑制细胞凋亡等三种方式促进胃癌的进展。
结论
circPGD 似乎在 H. pylori 相关胃癌中发挥作用，因此可能是一种可行的、新颖的治疗干预靶点。
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