一组来自家蚕的复杂早期绒毛膜DNA序列。

A complex set of early chorion DNA sequences from Bombyx mori.

作者信息

Eickbush T H, Rodakis G C, Lecanidou R, Kafatos F C

出版信息

Dev Biol. 1985 Dec;112(2):368-76. doi: 10.1016/0012-1606(85)90408-7.

Abstract

A follicular cDNA library from Bombyx mori (576 clones) was screened to obtain a representative sample of chorion sequences differentially expressed during the early period of choriogenesis. Ten selected sequences were characterized by Northern analysis and by dot blots of stage-specific RNA. Five of these sequences (represented in the library by 24 clones) had the developmental specificity and transcript size expected of early chorion components; two sequences (37 clones) had the developmental specificity expected of middle and late chorion components; and three sequences (16 clones) were presumed to be non-chorion, since they are constitutive or disappear at the beginning of choriogenesis. The five recovered early chorion cDNA components have been sequenced, and define three distinct subfamilies of the chorion B multigene family, and two C type families. The complexity of the early chorion genes in B. mori is discussed.

摘要

对家蚕卵泡cDNA文库（576个克隆）进行筛选，以获得在卵壳形成早期差异表达的卵壳序列的代表性样本。通过Northern分析和阶段特异性RNA的斑点印迹对10个选定序列进行了表征。其中5个序列（文库中有24个克隆代表）具有早期卵壳成分预期的发育特异性和转录本大小；2个序列（37个克隆）具有中期和晚期卵壳成分预期的发育特异性；3个序列（16个克隆）被推测为非卵壳序列，因为它们是组成型的或在卵壳形成开始时消失。已对5个回收的早期卵壳cDNA成分进行了测序，并确定了卵壳B多基因家族的三个不同亚家族和两个C型家族。讨论了家蚕早期卵壳基因的复杂性。