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运用固相微萃取、同时蒸馏萃取、气相色谱 - 嗅觉测量法、气相色谱 - 质谱联用、香气提取物稀释分析、气味活度值、重组 - 省略试验和感官评价对烤鸡肉中的关键香气化合物进行表征。

Characterization of key aroma compounds in roasted chicken using SPME, SAFE, GC-O, GC-MS, AEDA, OAV, recombination-omission tests, and sensory evaluation.

作者信息

Nie Ruotong, Zhang Chunjiang, Liu Huan, Wei Xiangru, Gao Rongmei, Shi Haonan, Zhang Dequan, Wang Zhenyu

机构信息

Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Integrated Laboratory of Processing Technology for Chinese Meat Dishes, Ministry of Agriculture and Rural Affairs, No. 2 Yuanmingyuan West Road, Haidian District, Beijing 100193, China.

出版信息

Food Chem X. 2024 Feb 17;21:101167. doi: 10.1016/j.fochx.2024.101167. eCollection 2024 Mar 30.

Abstract

Aroma compounds in the roasted breasts, thighs and skins of chicken were isolated by solvent-assisted flavor evaporation (SAFE), quantitated by gas chromatography-olfactometry-mass (GC-O-MS), analyzed by aroma extract dilution analysis (AEDA), and determined by recombination-omission tests and sensory evaluation. Forty-seven aroma compounds in total, including aldehydes, ketones, furans, pyrazines, and furanones, were selected by AEDA. Twenty-five compounds were selected as pivotal odorants (Odor Activity Value, OAV ≥ 1). Twenty aroma compounds significantly were identified by recombination and omission experiments. Anethole (fennel odor) was the highest OAV (> 1843). Hexanal (grassy) and ()-2,4-decadienal (meaty) were the most abundant aldehydes identified in roasted chicken. 1-octen-3-ol (mushroom), methanethiol (cabbage) and dimethyl trisulfide (areca, sulfur) were considered the key compounds of the breast and thighs of roasted chicken. Notably, furanone and pyrazines, 4-hydroxy-5-methyl-3()-furanone (caramel, sweet and burning odor), 3-ethyl-2,5-dimethylpyrazine (nutty, toasty) and 2,3-dimethyl-5-ethylpyrazine (nutty, toasty) had the most significant effect on roasted chicken odor, especially in the skin.

摘要

通过溶剂辅助风味蒸发(SAFE)法分离烤鸡肉胸、腿和皮中的香气化合物,采用气相色谱 - 嗅觉 - 质谱联用(GC - O - MS)进行定量分析,通过香气提取物稀释分析(AEDA)进行分析,并通过重组 - 遗漏试验和感官评价来确定。通过AEDA共筛选出47种香气化合物,包括醛类、酮类、呋喃类、吡嗪类和呋喃酮类。选择25种化合物作为关键气味物质(气味活性值,OAV≥1)。通过重组和遗漏实验明确鉴定出20种香气化合物。茴香脑(茴芹气味)的OAV最高(>1843)。己醛(青草味)和(E)-2,4 - 癸二烯醛(肉香味)是烤鸡肉中鉴定出的含量最丰富的醛类。1 - 辛烯 - 3 - 醇(蘑菇味)、甲硫醇(卷心菜味)和二甲基三硫醚(槟榔味、硫磺味)被认为是烤鸡肉胸和腿的关键化合物。值得注意的是,呋喃酮和吡嗪类,4 - 羟基 - 5 - 甲基 - 3(H)- 呋喃酮(焦糖味、甜味和焦糊味)、3 - 乙基 - 2,5 - 二甲基吡嗪(坚果味、烤香味)以及2,3 - 二甲基 - 5 - 乙基吡嗪(坚果味、烤香味)对烤鸡肉的气味影响最为显著,尤其是在鸡皮中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8f68/10900400/7369e01be938/ga1.jpg

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