评价γ 射线辐照对蜂胶抗氧化能力的影响。

Evaluation of gamma irradiation effects on antioxidant capacity of propolis.

机构信息

Institute of Catalysis, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bldg. 11, 1113, Sofia, Bulgaria.

Institute of Cryobiology and Food Technologies, Agricultural Academy, 1407, Sofia, Bulgaria.

出版信息

Appl Radiat Isot. 2024 May;207:111254. doi: 10.1016/j.apradiso.2024.111254. Epub 2024 Feb 24.

DOI:10.1016/j.apradiso.2024.111254

PMID:38430826

Abstract

The irradiation effects on antioxidant potential and on content of phenolic compounds of propolis ethanoic extracts were studied. It was found out that gamma treatment of samples with 2 and 10 kGy had a weak decreasing effect on the total phenolic content (TPC), while no change was observed in the propolis irradiated with 5 kGy. The antiradical activity of extracts was assessed by the DPPH free radical scavenging activity evaluated by Electron Paramagnetic Resonance (EPR) spectroscopy. The EPR results were in agreement with TPC. Some main phenolic compounds of the studied non-irradiated and irradiated samples were identified and compared by ultra-high performance liquid chromatography (UHPLC).

摘要

研究了辐照对蜂胶乙酸乙酯提取物抗氧化能力和酚类化合物含量的影响。结果发现，2 kGy 和 10 kGy 的γ处理对总酚含量（TPC）有微弱的降低作用，而 5 kGy 辐照的蜂胶则没有变化。通过电子顺磁共振（EPR）光谱评估 DPPH 自由基清除活性来评估提取物的抗自由基活性。EPR 结果与 TPC 一致。通过超高效液相色谱（UHPLC）对研究的未辐照和辐照样品的一些主要酚类化合物进行了鉴定和比较。