Özgür Yücel, Işık Reyhan, Orhan Bağnu, Berçik İnal Berrin, Özer Tanıl, Altun Şenel, Özcan Burcu
Bahçelievler State Hospital Anesthesiology and Reanimation Clinic, İstanbul, Turkey.
Department of Medical Biochemistry, Health Sciences University İstanbul Training and Research Hospital, İstanbul, Turkey.
Thorac Res Pract. 2024 Mar;25(2):62-67. doi: 10.5152/ThoracResPract.2024.23061.
In this study, the effect of tocilizumab (TCZ) on lung tissue in lung ischemia-reperfusion (I/R) injury in rats was investigated.
A total of 24 Wistar rats were divided into 4 equal groups, with 6 rats in each group: Left lung I/R was applied to I/R groups. In the I/R groups, the left lung hilum was clamped for 45 minutes, and then the clamp was removed and reperfused for 120 minutes. In the TCZ groups, 4 mg/kg and 8 mg/kg of TCZ were administered intraperitoneally to the rats 30 minutes before surgery.
The tumor necrosis factor-alpha mean value was not statistically significant between the groups (P = .091). Statistically significant results were observed between group I/R-TCZ (8 mg/kg) and group I/R for catalase. (P = .005). Statistically significant results were observed between group I/R-TCZ (8 mg/kg) and group I/R for malondialdehyde. (P = .009). The difference in total ischemia score between group I/R-TCZ (4 mg/kg) and group I/R-TCZ (8 mg/kg) and group I/R was statistically significant (P < .001). In terms of alveolar hemorrhage, there was a statistically significant difference between group I/R-TCZ (4 mg/kg) and group I/R-TCZ (8 mg/kg) and group I/R (P = .01 and P = .002, respectively). There was a statistically significant difference between group I/R-TCZ (8 mg/kg) and group I/R in terms of neutrophil accumulation (P = .01). In terms of interstitial edema, there was a statistically significant difference between group I/R-TCZ (4 mg/kg) and group I/R-TCZ (8 mg/kg) and group I/R (P = .006 and P = .001, respectively). In terms of pulmonary edema, there was a statistically significant difference between group I/R-TCZ (4 mg/kg) and group I/R-TCZ (8 mg/kg) and group I/R (P = .01 and P = .009, respectively).
Lung tissue may be affected by I/R injury and this damage can be reversed with the use of TCZ.
本研究旨在探讨托珠单抗（TCZ）对大鼠肺缺血再灌注（I/R）损伤中肺组织的影响。
将24只Wistar大鼠平均分为4组，每组6只：I/R组进行左肺I/R。在I/R组中，夹闭左肺门45分钟，然后松开夹子并再灌注120分钟。在TCZ组中，于手术前30分钟给大鼠腹腔注射4mg/kg和8mg/kg的TCZ。
各组间肿瘤坏死因子-α平均值无统计学意义（P = 0.091）。I/R-TCZ（8mg/kg）组与I/R组之间过氧化氢酶有统计学意义（P = 0.005）。I/R-TCZ（8mg/kg）组与I/R组之间丙二醛有统计学意义（P = 0.009）。I/R-TCZ（4mg/kg）组、I/R-TCZ（8mg/kg）组与I/R组之间总缺血评分差异有统计学意义（P < 0.001）。在肺泡出血方面，I/R-TCZ（4mg/kg）组、I/R-TCZ（8mg/kg）组与I/R组之间有统计学意义（分别为P = 0.01和P = 0.002）。I/R-TCZ（8mg/kg）组与I/R组在中性粒细胞积聚方面有统计学意义（P = 0.01）。在间质水肿方面，I/R-TCZ（4mg/kg）组、I/R-TCZ（8mg/kg）组与I/R组之间有统计学意义（分别为P = 0.006和P = 0.001）。在肺水肿方面，I/R-TCZ（4mg/kg）组、I/R-TCZ（8mg/kg）组与I/R组之间有统计学意义（分别为P = 0.01和P = 0.009）。
肺组织可能受I/R损伤影响，使用TCZ可逆转这种损伤。
