Northwest A&F Univ, Coll Food Sci & Engn, Shaanxi Engn Res Ctr Dairy Prod Qual Safety & Hlth, Yangling 712100, PR China.
Fuping County Inspection and Testing Center, Shaanxi Goat Milk Product Quality Supervision and Inspection Center, Fuping 711700, PR China.
Food Chem. 2024 Jul 30;447:138966. doi: 10.1016/j.foodchem.2024.138966. Epub 2024 Mar 7.
In this work, a simple, convenient and cost-effective colorimetric aptasensor was successfully constructed for the detection of antibiotic residues in raw milk based on the property that aptamer (Apt) synergistically enhances the catalase-like activity of MOF-235. Under optimised conditions, the proposed colorimetric aptasensor exhibited a wide detection range (15-1500 nM) with a low detection limit (6.92 nM). Furthermore, the proposed aptasensor demonstrated high selectivity, good resistance to interference and storage stability. The proposed aptasensor was validated by spiking recovery in camel milk, cow milk and goat milk with satisfactory recoveries, which demonstrated the great potential of the aptasensor for further application in real food samples, and also suggested that MOF-235 can be used as a potential universal platform to build a sensitive detection platform for other targets.
在这项工作中，成功构建了一种简单、方便且经济高效的比色适体传感器，用于基于适体（Apt）协同增强 MOF-235 过氧化物酶样活性的特性来检测生乳中的抗生素残留。在优化条件下，所提出的比色适体传感器具有较宽的检测范围（15-1500 nM）和较低的检测限（6.92 nM）。此外，该适体传感器表现出高选择性、良好的抗干扰性和存储稳定性。该适体传感器通过在骆驼奶、牛奶和羊奶中添加回收物进行验证，回收率令人满意，这表明该适体传感器在实际食品样品中的进一步应用具有巨大潜力，也表明 MOF-235 可用作构建其他目标敏感检测平台的潜在通用平台。
