Yang Shuai, Zhang Wei, Yang Bo, Feng Xin, Li Yiyang, Li Xiaolin, Liu Qin
Xinjiang Second Medical College, Xinjiang, China.
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin Co-funded by Xinjiang Production & Construction Corps and The Ministry of Science & Technology, Tarim University, Alar, China.
Front Microbiol. 2024 Mar 5;15:1330880. doi: 10.3389/fmicb.2024.1330880. eCollection 2024.
Due to the misuse of antibiotics, there is an increasing emergence and spread of multidrug-resistant (MDR) bacteria, leading to a human health crisis. To address clinical antibiotic resistance and prevent/control pathogenic microorganisms, the development of novel antibiotics is essential. This also offers a new approach to discovering valuable actinobacterial flora capable of producing natural bioactive products. In this study, we employed bioinformatics and macro-genome sequencing to collect 15 soil samples from three different locations in the Karamay Gobi region. First, we assessed the diversity of microorganisms in soil samples from different locations, analyzing the content of bacteria, archaea, actinomycetes, and fungi. The biodiversity of soil samples from outside the Gobi was found to be higher than that of soil samples from within and in the center of the Gobi. Second, through microbial interaction network analysis, we identified actinomycetes as the dominant group in the system. We have identified the top four antibiotic genes, such as , , A (58), and B, by CARD. These genes are associated with peptide antibiotics, disinfecting agents and antiseptics, tetracycline antibiotics, and macrolide antibiotics. In addition, we also obtained 40 other antibiotic-related genes through CARD alignment. Through in-depth analysis of desert soil samples, we identified several unstudied microbial species belonging to different families, including , , , , , , , and . These species have the capability to produce peptide antibiotics, macrolide antibiotics, and tetracycline antibiotics, as well as disinfectants and preservatives. This study provides valuable theoretical support for future in-depth research.
由于抗生素的滥用，多重耐药（MDR）细菌不断出现并传播，引发了人类健康危机。为应对临床抗生素耐药性并预防/控制病原微生物，开发新型抗生素至关重要。这也为发现能够产生天然生物活性产物的有价值放线菌菌群提供了新途径。在本研究中，我们运用生物信息学和宏基因组测序技术，从克拉玛依戈壁地区的三个不同地点采集了15份土壤样本。首先，我们评估了不同地点土壤样本中微生物的多样性，分析了细菌、古菌、放线菌和真菌的含量。发现戈壁外土壤样本的生物多样性高于戈壁内和戈壁中心的土壤样本。其次，通过微生物相互作用网络分析，我们确定放线菌是该系统中的优势菌群。我们通过CARD鉴定出了前四个抗生素基因，如 、 、A（58）和B。这些基因与肽类抗生素、消毒剂和防腐剂、四环素类抗生素以及大环内酯类抗生素有关。此外，我们还通过CARD比对获得了40个其他与抗生素相关的基因。通过对沙漠土壤样本的深入分析，我们鉴定出了几个属于不同科的未被研究的微生物物种，包括 、 、 、 、 、 、 和 。这些物种具有产生肽类抗生素、大环内酯类抗生素和四环素类抗生素以及消毒剂和防腐剂的能力。本研究为未来的深入研究提供了有价值的理论支持。
