胃癌上皮-间质转化体外模型的设计
Design of an In Vitro Model for Epithelial-to-Mesenchymal Transition in Gastric Cancer.
作者信息
Zhang Yuanhui, Bi Ling, Li Quanyao, Yao Liqiu, Wang Xiao, Liu Hui, Shi Jun
机构信息
Department of Oncology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200437, China.
Department of Traditional Chinese Medicine, Shanghai Fourth People's Hospital Affiliated to Tongji University of Medicine, Shanghai, 200434, China.
出版信息
Biochem Genet. 2025 Feb;63(1):698-712. doi: 10.1007/s10528-024-10668-x. Epub 2024 Mar 20.
Epithelial-to-mesenchymal transition (EMT) is a developmental program that plays a vital role in gastric cancer, including aspects of tumor progression, the metastatic process, and resistance to treatment. Here, we have designed an in vitro model that mimics the features of EMT as observed in gastric cancer. The results showed that both migration and invasion were enhanced in gastric cancer cells with Brachyury overexpression. Additionally, the expression of IL-8 increased, while IL-8RA and IL-8RB levels significantly decreased in the in vitro model. Overall, the in vitro model offers an opportunity to study these phenomena relevant to EMT as they may occur in vivo in gastric cancer, as well as potential drug interactions that could interfere with these processes.
上皮-间质转化（EMT）是一种发育程序，在胃癌中起着至关重要的作用，包括肿瘤进展、转移过程和治疗抵抗等方面。在此，我们设计了一种体外模型，该模型模拟了胃癌中观察到的EMT特征。结果表明，过表达Brachyury的胃癌细胞的迁移和侵袭能力均增强。此外，在体外模型中，IL-8的表达增加，而IL-8RA和IL-8RB水平显著降低。总体而言，该体外模型为研究这些与EMT相关的现象提供了一个机会，因为它们可能在胃癌体内发生，同时也为研究可能干扰这些过程的潜在药物相互作用提供了机会。
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