荞麦胚胎拯救技术。

Embryo Rescue Technique in Buckwheat Fagopyrum esculentum Moench.

机构信息

Federal State Budgetary Scientific Institution, Federal Scientific Center of Legumes and Groat Crops, Orel oblast, Russia.

出版信息

Methods Mol Biol. 2024;2791:89-96. doi: 10.1007/978-1-0716-3794-4_9.

DOI:10.1007/978-1-0716-3794-4_9

PMID:38532095

Abstract

Culture of immature buckwheat embryos is an excellent explant for tissue culture and regeneration in vitro, as well as a method of embryo rescue in case of fruit abortion especially in interspecific crosses. Here, we describe the method of immature embryo rescue using a differentiated approach to different age groups. The method involves the development of embryos along the path of direct embryogenesis bypassing the callus stage.

摘要

未成熟荞麦胚胎的培养是组织培养和体外再生的极佳外植体，也是果实败育特别是种间杂交时胚胎挽救的方法。在这里，我们描述了一种使用不同年龄组分化方法进行未成熟胚胎挽救的方法。该方法涉及通过绕过愈伤组织阶段沿直接胚胎发生途径发育胚胎。